Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms20205174
Title: Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering
Authors: HARIHARAN EZHILARASU 
Ramalingam, Raghavendra
CHETNA DHAND 
RAJAMANI LAKSHMINARAYANAN 
Sadiq, Asif
CHINNASAMY GANDHIMATHI 
SEERAM RAMAKRISHNA 
BAY BOON HUAT 
JAYARAMA REDDY VENUGOPAL 
SRINIVASAN DINESH KUMAR 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Multidisciplinary
Chemistry
electrospinning
nanofibers
curcumin
aloe vera
tetracycline hydrochloride
fibroblasts
wound healing
Issue Date: 2-Oct-2019
Publisher: MDPI
Citation: HARIHARAN EZHILARASU, Ramalingam, Raghavendra, CHETNA DHAND, RAJAMANI LAKSHMINARAYANAN, Sadiq, Asif, CHINNASAMY GANDHIMATHI, SEERAM RAMAKRISHNA, BAY BOON HUAT, JAYARAMA REDDY VENUGOPAL, SRINIVASAN DINESH KUMAR (2019-10-02). Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20 (20). ScholarBank@NUS Repository. https://doi.org/10.3390/ijms20205174
Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-ε-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc diffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering.
Source Title: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
URI: https://scholarbank.nus.edu.sg/handle/10635/164327
ISSN: 1661-6596
1422-0067
DOI: 10.3390/ijms20205174
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