Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms20205135
Title: Osteogenic Differentiation of Mesenchymal Stem Cells with Silica-Coated Gold Nanoparticles for Bone Tissue Engineering
Authors: CHINNASAMY GANDHIMATHI 
QUEK YING JIE 
HARIHARAN EZHILARASU 
SEERAM RAMAKRISHNA 
BAY BOON HUAT 
SRINIVASAN DINESH KUMAR 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Multidisciplinary
Chemistry
pcl
silk fibroin
silica-coated gold nanoparticles
nanofibrous scaffolds
mineralization
bone tissue engineering
ELECTROSPUN
SCAFFOLDS
NANOFIBERS
DELIVERY
PEPTIDE
Issue Date: 2-Oct-2019
Publisher: MDPI
Citation: CHINNASAMY GANDHIMATHI, QUEK YING JIE, HARIHARAN EZHILARASU, SEERAM RAMAKRISHNA, BAY BOON HUAT, SRINIVASAN DINESH KUMAR (2019-10-02). Osteogenic Differentiation of Mesenchymal Stem Cells with Silica-Coated Gold Nanoparticles for Bone Tissue Engineering. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20 (20). ScholarBank@NUS Repository. https://doi.org/10.3390/ijms20205135
Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Multifunctional nanofibrous scaffolds for effective bone tissue engineering (BTE) application must incorporate factors to promote neovascularization and tissue regeneration. In this study, silica-coated gold nanoparticles Au(SiO2) were tested for their ability to promote differentiation of human mesenchymal stem cells (hMSCs) into osteoblasts. Biocompatible poly-ε-caprolactone (PCL), PCL/silk fibroin (SF) and PCL/SF/Au(SiO2) loaded nanofibrous scaffolds were first fabricated by an electrospinning method. Electrospun nanofibrous scaffolds were characterized for fiber architecture, porosity, pore size distribution, fiber wettability and the relevant mechanical properties using field emission scanning electron microscopy (FESEM), porosimetry, determination of water contact angle, measurements by a surface analyzer and tabletop tensile-tester measurements. FESEM images of the scaffolds revealed beadless, porous, uniform fibers with diameters in the range of 164 ± 18.65 nm to 215 ± 32.12 nm and porosity of around 88–92% and pore size distribution around 1.45–2.35 µm. Following hMSCs were cultured on the composite scaffolds. Cell-scaffold interaction, morphology and proliferation of were analyzed by FESEM analysis, MTS (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) and CMFDA (5-choromethyl fluorescein acetate) dye assays. Osteogenic differentiation of MSCs into osteogenic cells were determined by alkaline phosphatase (ALP) activity, mineralization by alizarin red S (ARS) staining and osteocalcin expression by immunofluorescence staining. The results revealed that the addition of SF and Au(SiO2) to PCL scaffolds enhanced the mechanical strength, interconnecting porous structure and surface roughness of the scaffolds. This, in turn, led to successful osteogenic differentiation of hMSCs with improved cell adhesion, proliferation, differentiation, mineralization and expression of pro-osteogenic cellular proteins. This provides huge support for Au(SiO2) as a suitable material in BTE.
Source Title: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
URI: https://scholarbank.nus.edu.sg/handle/10635/164326
ISSN: 1661-6596
1422-0067
DOI: 10.3390/ijms20205135
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