Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/163919
Title: Differential expression of cytokines in the rat heart in response to sustained volume overload
Authors: Dai, RP
Dheen, ST 
He, BP
Tay, SSW 
Keywords: Science & Technology
Life Sciences & Biomedicine
Cardiac & Cardiovascular Systems
Cardiovascular System & Cardiology
hypertrophy
remodeling
cytokines
volume overload
CONTRACTILE DYSFUNCTION
CARDIAC MYOCYTES
HYPERTROPHIED MYOCARDIUM
GENE-EXPRESSION
ANGIOTENSIN-II
NITRIC-OXIDE
FAILURE
INTERLEUKIN-6
PRESSURE
STRESS
Issue Date: 1-Oct-2004
Publisher: WILEY
Citation: Dai, RP, Dheen, ST, He, BP, Tay, SSW (2004-10-01). Differential expression of cytokines in the rat heart in response to sustained volume overload. EUROPEAN JOURNAL OF HEART FAILURE 6 (6) : 693-703. ScholarBank@NUS Repository.
Abstract: Objective: The present study aimed to investigate whether sustained volume overload is capable of inducing persistent upregulation of cardiac cytokines including tumor necrosis factor alpha (TNF)-α, interleukin (IL)-1β, interleukin (IL)-6 and transforming growth factor (TGF)-β1. Methods and Results: Volume overload-induced heart hypertrophy in rats was established by aortacaval fistula, and the cardiac cytokines were measured in the myocardium from 1 to 4 weeks after operation. In the post-fistula rats, cardiac IL-1β and IL-6 gene and protein levels were upregulated throughout the time of measurement. Immunohistochemistry demonstrated that IL-1β and IL-6 immunoreactive cells were widely distributed in the myocardium in the earlier time intervals, and mainly localized in the regions close to the endocardium in the later time intervals. The cardiac IL-1β immunoreactive cells were mainly localized in the blood vessels whereas the IL-6 positive cells were composed of non-myocytes and cardiomyocytes. TGF-β1 positive staining was increased in the myocardium up to 3 weeks after aortacaval fistula and then decreased to basal levels thereafter. In contrast to the activation of cardiac IL-1β and IL-6 in response to volume overload, TNF-α expression appeared unaltered in response to sustained volume overload in the transcription and protein levels. Conclusion: The results of the present study indicate that sustained volume overload is capable of inducing persistent upregulation of some cardiac cytokines. In addition, the differential expressions of TNF-α, IL-1β and IL-6 suggest that the induction of IL-6 and IL-1β is independent of TNF-α mediated pathways in this animal model. © 2003 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.
Source Title: EUROPEAN JOURNAL OF HEART FAILURE
URI: https://scholarbank.nus.edu.sg/handle/10635/163919
ISSN: 13889842
18790844
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