Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/163916
Title: Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid
Authors: Kaur, C 
Sivakumar, V 
Dheen, ST 
Ling, EA 
Keywords: All-trans-retinoic acid
Corpus callosum
Insulin-like growth factor I and II
Lipopolysaccharide
Microglial cells
Issue Date: 1-Jan-2006
Publisher: ELSEVIER
Citation: Kaur, C, Sivakumar, V, Dheen, ST, Ling, EA (2006-01-01). Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid. NEUROSCIENCE 138 (4) : 1233-1244. ScholarBank@NUS Repository.
Abstract: Insulin-like growth factors I and II are known to regulate the development of the CNS. We examined the developmental changes in insulin-like growth factor I and insulin-like growth factor II expression in the postnatal rat corpus callosum. Insulin-like growth factor I and insulin-like growth factor II mRNA expression increased at 3 days as compared with 1 day whereas the protein expression increased up to 7 days. Insulin-like growth factor I and insulin-like growth factor II immunoexpression was specifically localized in round cells confirmed by double immunofluorescence with OX-42 to be the amoeboid microglial cells. Insulin-like growth factor I expression was observed up to 7 days in amoeboid microglial cells while insulin-like growth factor II expression was detected in 1-3 day old rats. Exposure of primary rat microglial cell cultures to lipopolysaccharide increased insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression significantly along with their immunoexpression in microglial cells. The lipopolysaccharide-induced increase in insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression was significantly decreased with all-trans-retinoic acid. We conclude that insulin-like growth factor I and insulin-like growth factor II expression in amoeboid microglial cells in the developing brain is related to their activation. Once the activation is inhibited, either by transformation of the amoeboid microglial cells into ramified microglia regarded as resting cells or as shown by the effect of all-trans-retinoic acid administration, insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression is downregulated.
Source Title: NEUROSCIENCE
URI: https://scholarbank.nus.edu.sg/handle/10635/163916
ISSN: 03064522
18737544
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