Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/163916
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dc.titleInsulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid
dc.contributor.authorKaur, C
dc.contributor.authorSivakumar, V
dc.contributor.authorDheen, ST
dc.contributor.authorLing, EA
dc.date.accessioned2020-01-20T06:41:21Z
dc.date.available2020-01-20T06:41:21Z
dc.date.issued2006-01-01
dc.identifier.citationKaur, C, Sivakumar, V, Dheen, ST, Ling, EA (2006-01-01). Insulin-like growth factor I and II expression and modulation in amoeboid microglial cells by lipopolysaccharide and retinoic acid. NEUROSCIENCE 138 (4) : 1233-1244. ScholarBank@NUS Repository.
dc.identifier.issn03064522
dc.identifier.issn18737544
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/163916
dc.description.abstractInsulin-like growth factors I and II are known to regulate the development of the CNS. We examined the developmental changes in insulin-like growth factor I and insulin-like growth factor II expression in the postnatal rat corpus callosum. Insulin-like growth factor I and insulin-like growth factor II mRNA expression increased at 3 days as compared with 1 day whereas the protein expression increased up to 7 days. Insulin-like growth factor I and insulin-like growth factor II immunoexpression was specifically localized in round cells confirmed by double immunofluorescence with OX-42 to be the amoeboid microglial cells. Insulin-like growth factor I expression was observed up to 7 days in amoeboid microglial cells while insulin-like growth factor II expression was detected in 1-3 day old rats. Exposure of primary rat microglial cell cultures to lipopolysaccharide increased insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression significantly along with their immunoexpression in microglial cells. The lipopolysaccharide-induced increase in insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression was significantly decreased with all-trans-retinoic acid. We conclude that insulin-like growth factor I and insulin-like growth factor II expression in amoeboid microglial cells in the developing brain is related to their activation. Once the activation is inhibited, either by transformation of the amoeboid microglial cells into ramified microglia regarded as resting cells or as shown by the effect of all-trans-retinoic acid administration, insulin-like growth factor I and insulin-like growth factor II mRNA and protein expression is downregulated.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.neuroscience.2005.12.025
dc.language.isoen
dc.publisherELSEVIER
dc.sourceElements
dc.subjectAll-trans-retinoic acid
dc.subjectCorpus callosum
dc.subjectInsulin-like growth factor I and II
dc.subjectLipopolysaccharide
dc.subjectMicroglial cells
dc.typeArticle
dc.date.updated2020-01-17T07:48:59Z
dc.contributor.departmentDEPT OF ANATOMY
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.sourcetitleNEUROSCIENCE
dc.description.volume138
dc.description.issue4
dc.description.page1233-1244
dc.description.codenNRSCD
dc.identifier.isiut000236504100019
dc.description.placeUNITED KINGDOM
dc.published.statePublished
dc.grant.idR181-000-065-112
dc.grant.fundingagencyNational University of Singapore
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