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https://doi.org/10.1371/journal.pone.0022978
Title: | Probiotics modulate intestinal expression of nuclear receptor and provide Counter-Regulatory signals to Inflammation-Driven adipose tissue activation | Authors: | Mencarelli A. Distrutti E. Renga B. D'Amore C. Cipriani S. Palladino G. Donini A. Ricci P. Fiorucci S. |
Keywords: | adiponectin cell nucleus receptor ciprofloxacin farnesoid X receptor gamma interferon interleukin 6 leptin liver X receptor metronidazole peroxisome proliferator activated receptor alpha peroxisome proliferator activated receptor gamma pregnane X receptor probiotic agent tumor necrosis factor alpha tumor necrosis factor alpha inhibitor VSL3 cell receptor cytokine farnesoid X receptor farnesoid X-activated receptor liver X receptor messenger RNA orphan nuclear receptor peroxisome proliferator activated receptor gamma pregnane X receptor probiotic agent steroid receptor trinitrobenzenesulfonic acid abdominal abscess abdominal fat adipose tissue adult animal experiment animal model animal tissue antiinflammatory activity article clinical article clinical feature colitis colon cancer concentration response controlled study Crohn disease disease course enteritis enzyme activation female human human tissue immunomodulation inflammation intestine cell intestine fistula male mouse nonhuman protein expression protein function protein localization protein secretion regulatory mechanism signal transduction tissue culture tissue level treatment duration animal cell culture chemically induced disorder colitis drug effect enzyme immunoassay enzyme linked immunosorbent assay genetic transcription genetics intestine mesentery metabolism pathology pathophysiology reverse transcription polymerase chain reaction Western blotting Animalia Mus Rodentia Adipose Tissue Adult Animals Blotting, Western Cells, Cultured Colitis Cytokines Enzyme-Linked Immunosorbent Assay Female Humans Immunoenzyme Techniques Inflammation Intestines Male Mesentery Mice Orphan Nuclear Receptors PPAR gamma Probiotics Receptors, Cytoplasmic and Nuclear Receptors, Steroid Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Signal Transduction Transcription, Genetic Trinitrobenzenesulfonic Acid |
Issue Date: | 2011 | Citation: | Mencarelli A., Distrutti E., Renga B., D'Amore C., Cipriani S., Palladino G., Donini A., Ricci P., Fiorucci S. (2011). Probiotics modulate intestinal expression of nuclear receptor and provide Counter-Regulatory signals to Inflammation-Driven adipose tissue activation. PLoS ONE 6 (7) : e22978. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0022978 | Rights: | Attribution 4.0 International | Abstract: | Background: Adipocytes from mesenteric white adipose tissue amplify the inflammatory response and participate in inflammation-driven immune dysfunction in Crohn's disease by releasing proinflammatory mediators. Peroxisome proliferator-activated receptors (PPAR)-? and -?, pregnane x receptor (PXR), farnesoid x receptor (FXR) and liver x-receptor (LXR) are ligand-activated nuclear receptor that provide counter-regulatory signals to dysregulated immunity and modulates adipose tissue. Aims: To investigate the expression and function of nuclear receptors in intestinal and adipose tissues in a rodent model of colitis and mesenteric fat from Crohn's patients and to investigate their modulation by probiotics. Methods: Colitis was induced by TNBS administration. Mice were administered vehicle or VSL#3, daily for 10 days. Abdominal fat explants obtained at surgery from five Crohn's disease patients and five patients with colon cancer were cultured with VSL#3 medium. Results: Probiotic administration attenuated development of signs and symptoms of colitis, reduced colonic expression of TNF?, IL-6 and IFN? and reserved colonic downregulation of PPAR?, PXR and FXR caused by TNBS. Mesenteric fat depots isolated from TNBS-treated animals had increased expression of inflammatory mediators along with PPAR?, FXR, leptin and adiponectin. These changes were prevented by VSL#3. Creeping fat and mesenteric adipose tissue from Crohn's patients showed a differential expression of PPAR? and FXR with both tissue expressing high levels of leptin. Exposure of these tissues to VSL#3 medium abrogates leptin release. Conclusions: Mesenteric adipose tissue from rodent colitis and Crohn's disease is metabolically active and shows inflammation-driven regulation of PPAR?, FXR and leptin. Probiotics correct the inflammation-driven metabolic dysfunction. © 2011 Mencarelli et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/162037 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0022978 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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