Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0024573
Title: An inhibitory effect of extracellular Ca 2+ on Ca 2+-dependent exocytosis
Authors: Xiong W.
Liu T.
Wang Y.
Chen X.
Sun L.
Guo N.
Zheng H.
Zheng L.
Ruat M.
Han W. 
Zhang C.X.
Zhou Z.
Keywords: antibiotic g 418
cadmium
caffeine
calcium ion
calcium sensing receptor
magnesium ion
neomycin
caffeine
calcimimetic agent
calcium
animal cell
animal tissue
article
calcium transport
cell vacuole
chromaffin cell
concentration (parameters)
exocytosis
extracellular calcium
inhibition kinetics
nerve cell
nonhuman
photolysis
rat
spinal ganglion
animal
cell membrane
cytology
drug effect
extracellular space
metabolism
Wistar rat
Rattus
Animals
Caffeine
Calcimimetic Agents
Calcium
Cell Membrane
Chromaffin Cells
Exocytosis
Extracellular Space
Ganglia, Spinal
Neurons
Photolysis
Rats
Rats, Wistar
Issue Date: 2011
Citation: Xiong W., Liu T., Wang Y., Chen X., Sun L., Guo N., Zheng H., Zheng L., Ruat M., Han W., Zhang C.X., Zhou Z. (2011). An inhibitory effect of extracellular Ca 2+ on Ca 2+-dependent exocytosis. PLoS ONE 6 (10) : e24573. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0024573
Rights: Attribution 4.0 International
Abstract: Aim: Neurotransmitter release is elicited by an elevation of intracellular Ca 2+ concentration ([Ca 2+] i). The action potential triggers Ca 2+ influx through Ca 2+ channels which causes local changes of [Ca 2+] i for vesicle release. However, any direct role of extracellular Ca 2+ (besides Ca 2+ influx) on Ca 2+-dependent exocytosis remains elusive. Here we set out to investigate this possibility on rat dorsal root ganglion (DRG) neurons and chromaffin cells, widely used models for studying vesicle exocytosis. Results: Using photolysis of caged Ca 2+ and caffeine-induced release of stored Ca 2+, we found that extracellular Ca 2+ inhibited exocytosis following moderate [Ca 2+] i rises (2-3 ?M). The IC 50 for extracellular Ca 2+ inhibition of exocytosis (ECIE) was 1.38 mM and a physiological reduction (~30%) of extracellular Ca 2+ concentration ([Ca 2+] o) significantly increased the evoked exocytosis. At the single vesicle level, quantal size and release frequency were also altered by physiological [Ca 2+] o. The calcimimetics Mg 2+, Cd 2+, G418, and neomycin all inhibited exocytosis. The extracellular Ca 2+-sensing receptor (CaSR) was not involved because specific drugs and knockdown of CaSR in DRG neurons did not affect ECIE. Conclusion/Significance: As an extension of the classic Ca 2+ hypothesis of synaptic release, physiological levels of extracellular Ca 2+ play dual roles in evoked exocytosis by providing a source of Ca 2+ influx, and by directly regulating quantal size and release probability in neuronal cells. © 2011 Xiong et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/162028
ISSN: 19326203
DOI: 10.1371/journal.pone.0024573
Rights: Attribution 4.0 International
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