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https://doi.org/10.1371/journal.pntd.0002891
| Title: | The Interface between Methyltransferase and Polymerase of NS5 Is Essential for Flavivirus Replication | Authors: | Li X.-D. Shan C. Deng C.-L. Ye H.-Q. Shi P.-Y. Yuan Z.-M. Gong P. Zhang B. |
Keywords: | complementary DNA methyltransferase nonstructural protein 5 RNA directed RNA polymerase virus RNA DNA directed RNA polymerase methyltransferase NS5 protein, flavivirus virus protein animal cell article controlled study electroporation enzyme activity Flavivirus infection gene mutation gene sequence genetic transfection immunofluorescence microscopy immunofluorescence test Japanese encephalitis virus luciferase assay molecular interaction nonhuman replicon reverse transcription polymerase chain reaction RNA extraction RNA replication RNA transcription virus genome virus mutant virus plaque virus replication amino acid sequence cell line chemical phenomena chemistry Flavivirus genetics molecular genetics nucleotide sequence physiology sequence alignment virus replication Amino Acid Sequence Cell Line Conserved Sequence DNA-Directed RNA Polymerases Flavivirus Hydrophobic and Hydrophilic Interactions Methyltransferases Molecular Sequence Data Sequence Alignment Viral Nonstructural Proteins Virus Replication |
Issue Date: | 2014 | Citation: | Li X.-D., Shan C., Deng C.-L., Ye H.-Q., Shi P.-Y., Yuan Z.-M., Gong P., Zhang B. (2014). The Interface between Methyltransferase and Polymerase of NS5 Is Essential for Flavivirus Replication. PLoS Neglected Tropical Diseases 8 (5) : e2891. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pntd.0002891 | Rights: | Attribution 4.0 International | Abstract: | The flavivirus NS5 harbors both a methyltransferase (MTase) and an RNA-dependent RNA polymerase (RdRP). Both enzyme activities of NS5 are critical for viral replication. Recently, the full-length NS5 crystal structure of Japanese encephalitis virus reveals a conserved MTase-RdRP interface that features two conserved components: a six-residue hydrophobic network and a GTR sequence. Here we showed for the first time that these key interface components are essential for flavivirus replication by various reverse genetics approaches. Interestingly, some replication-impaired variants generated a common compensatory NS5 mutation outside the interface (L322F), providing novel routes to further explore the crosstalk between MTase and RdRP. ? 2014 Li et al. | Source Title: | PLoS Neglected Tropical Diseases | URI: | https://scholarbank.nus.edu.sg/handle/10635/161951 | ISSN: | 19352727 | DOI: | 10.1371/journal.pntd.0002891 | Rights: | Attribution 4.0 International |
| Appears in Collections: | Elements Staff Publications |
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