Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.ppat.1004548
Title: Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection
Authors: Cerny D.
Haniffa M.
Shin A.
Bigliardi P. 
Tan B.K. 
Lee B.
Poidinger M. 
Tan E.Y.
Ginhoux F. 
Fink K.
Keywords: beta interferon
macrophage inflammatory protein 3beta
RANTES
STAT protein
virus RNA
animal cell
antigen binding
antigen presenting cell
apoptosis
Article
cell migration
cell proliferation assay
cell survival
chemotaxis assay
controlled study
data analysis
dengue
Dengue virus
disease predisposition
flow cytometry
human
human tissue
immune response
lymph node
macrophage
mouse
nonhuman
protein determination
protein expression
real time polymerase chain reaction
reverse transcription polymerase chain reaction
skin
virus load
animal
immunology
knockout mouse
Langerhans cell
pathology
virology
Dengue virus
Mus
Animals
Dengue
Dengue Virus
Humans
Langerhans Cells
Macrophages
Mice
Mice, Knockout
Issue Date: 2014
Citation: Cerny D., Haniffa M., Shin A., Bigliardi P., Tan B.K., Lee B., Poidinger M., Tan E.Y., Ginhoux F., Fink K. (2014). Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. PLoS Pathogens 10 (12) : Jan-15. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.1004548
Abstract: Dengue is a growing global concern with 390 million people infected each year. Dengue virus (DENV) is transmitted by mosquitoes, thus host cells in the skin are the first point of contact with the virus. Human skin contains several populations of antigen-presenting cells which could drive the immune response to DENV in vivo: epidermal Langerhans cells (LCs), three populations of dermal dendritic cells (DCs), and macrophages. Using samples of normal human skin we detected productive infection of CD14+ and CD1c+ DCs, LCs and dermal macrophages, which was independent of DC-SIGN expression. LCs produced the highest viral titers and were less sensitive to IFN-?. Nanostring gene expression data showed significant up-regulation of IFN-?, STAT-1 and CCL5 upon viral exposure in susceptible DC populations. In mice infected intra-dermally with DENV we detected parallel populations of infected DCs originating from the dermis and migrating to the skin-draining lymph nodes. Therefore dermal DCs may simultaneously facilitate systemic spread of DENV and initiate the adaptive anti-viral immune response. ? 2014 Cerny et al.
Source Title: PLoS Pathogens
URI: https://scholarbank.nus.edu.sg/handle/10635/161944
ISSN: 15537366
DOI: 10.1371/journal.ppat.1004548
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_ppat_1004548.pdf2.48 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

55
checked on Jul 5, 2020

Page view(s)

60
checked on Jul 2, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.