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https://doi.org/10.1371/journal.ppat.1004548
Title: | Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection | Authors: | Cerny D. Haniffa M. Shin A. Bigliardi P. Tan B.K. Lee B. Poidinger M. Tan E.Y. Ginhoux F. Fink K. |
Keywords: | beta interferon macrophage inflammatory protein 3beta RANTES STAT protein virus RNA animal cell antigen binding antigen presenting cell apoptosis Article cell migration cell proliferation assay cell survival chemotaxis assay controlled study data analysis dengue Dengue virus disease predisposition flow cytometry human human tissue immune response lymph node macrophage mouse nonhuman protein determination protein expression real time polymerase chain reaction reverse transcription polymerase chain reaction skin virus load animal immunology knockout mouse Langerhans cell pathology virology Dengue virus Mus Animals Dengue Dengue Virus Humans Langerhans Cells Macrophages Mice Mice, Knockout |
Issue Date: | 2014 | Citation: | Cerny D., Haniffa M., Shin A., Bigliardi P., Tan B.K., Lee B., Poidinger M., Tan E.Y., Ginhoux F., Fink K. (2014). Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. PLoS Pathogens 10 (12) : 1-15. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.1004548 | Rights: | Attribution 4.0 International | Abstract: | Dengue is a growing global concern with 390 million people infected each year. Dengue virus (DENV) is transmitted by mosquitoes, thus host cells in the skin are the first point of contact with the virus. Human skin contains several populations of antigen-presenting cells which could drive the immune response to DENV in vivo: epidermal Langerhans cells (LCs), three populations of dermal dendritic cells (DCs), and macrophages. Using samples of normal human skin we detected productive infection of CD14+ and CD1c+ DCs, LCs and dermal macrophages, which was independent of DC-SIGN expression. LCs produced the highest viral titers and were less sensitive to IFN-?. Nanostring gene expression data showed significant up-regulation of IFN-?, STAT-1 and CCL5 upon viral exposure in susceptible DC populations. In mice infected intra-dermally with DENV we detected parallel populations of infected DCs originating from the dermis and migrating to the skin-draining lymph nodes. Therefore dermal DCs may simultaneously facilitate systemic spread of DENV and initiate the adaptive anti-viral immune response. ? 2014 Cerny et al. | Source Title: | PLoS Pathogens | URI: | https://scholarbank.nus.edu.sg/handle/10635/161944 | ISSN: | 15537366 | DOI: | 10.1371/journal.ppat.1004548 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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