Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.ppat.0030060
Title: A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells
Authors: Song J.
Duncan M.J.
Li G.
Chan C.
Grady R.
Stapleton A.
Abraham S.N. 
Keywords: adenylate cyclase
cyclic AMP
cyclic AMP responsive element binding protein binding protein
cytokine
immunoglobulin enhancer binding protein
interleukin 6
lipopolysaccharide
toll like receptor 4
transcription factor
adenylate cyclase
bacterial RNA
calcium
CREBBP protein, human
cyclic AMP
cyclic AMP responsive element binding protein binding protein
immunoglobulin enhancer binding protein
lipopolysaccharide
TLR4 protein, human
unclassified drug
article
bladder epithelium
calcium cell level
cell motility
controlled study
epithelium cell
Escherichia coli
human
human cell
nonhuman
protein analysis
protein expression
protein phosphorylation
protein secretion
signal transduction
bladder
cytology
Enterobacter infection
Escherichia coli
fimbria
genetics
immunology
metabolism
microbiology
phosphorylation
signal transduction
Escherichia coli
Negibacteria
Adenylate Cyclase
Calcium
CREB-Binding Protein
Cyclic AMP
Epithelial Cells
Escherichia coli
Escherichia coli Infections
Fimbriae, Bacterial
Humans
Interleukin-6
Lipopolysaccharides
NF-kappa B
Phosphorylation
RNA, Bacterial
Signal Transduction
Toll-Like Receptor 4
Urinary Bladder
Issue Date: 2007
Citation: Song J., Duncan M.J., Li G., Chan C., Grady R., Stapleton A., Abraham S.N. (2007). A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. PLoS Pathogens 3 (4) : 541-552. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.0030060
Rights: Attribution 4.0 International
Abstract: The vigorous cytokine response of immune cells to Gram-negative bacteria is primarily mediated by a recognition molecule, Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide (LPS) and initiates a series of intracellular NF-?B-associated signaling events. Recently, bladder epithelial cells (BECs) were reported to express TLR4 and to evoke a vigorous cytokine response upon exposure to LPS. We examined intracellular signaling events in human BECs leading to the production of IL-6, a major urinary cytokine, following activation by Escherichia coli and isolated LPS. We observed that in addition to the classical NF-?B-associated pathway, TLR4 triggers a distinct and more rapid signaling response involving, sequentially, Ca 2+, adenylyl cyclase 3-generated cAMP, and a transcriptional factor, cAMP response element-binding protein. This capacity of BECs to mobilize secondary messengers and evoke a more rapid IL-6 response might be critical in their role as first responders to microbial challenge in the urinary tract. � 2007 Song et al.
Source Title: PLoS Pathogens
URI: https://scholarbank.nus.edu.sg/handle/10635/161867
ISSN: 15537366
DOI: 10.1371/journal.ppat.0030060
Rights: Attribution 4.0 International
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