Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0113445
Title: Characterization of the runx gene family in a jawless vertebrate, the Japanese lamprey (lethenteron japonicum)
Authors: Nah G.S.S. 
Tay B.-H.
Brenner S. 
Osato M. 
Venkatesh B. 
Keywords: complementary DNA
microRNA
microRNA 130b
microRNA 131ab
microRNA 27
transcription factor RUNX
transcription factor RUNX1
transcription factor RUNX2
transcription factor RUNX3
unclassified drug
3' untranslated region
fish protein
isoprotein
microRNA
protein subunit
transcription factor RUNX
3' untranslated region
Article
binding site
cyclostome
gene
gene amplification
gene expression profiling
genetic organization
genetic trait
genome
lamprey
molecular cloning
molecular evolution
nonhuman
nucleotide sequence
orthology
phylogeny
promoter region
protein domain
protein motif
reverse transcription polymerase chain reaction
RunxA gene
RunxB gene
RunxC gene
sequence analysis
synteny
alternative RNA splicing
amino acid sequence
animal
biological model
classification
DNA sequence
exon
female
genetics
intron
male
metabolism
molecular genetics
multigene family
Petromyzon
protein subunit
sequence homology
Lethenteron japonicum
Vertebrata
3' Untranslated Regions
Alternative Splicing
Amino Acid Sequence
Animals
Binding Sites
Core Binding Factor alpha Subunits
Evolution, Molecular
Exons
Female
Fish Proteins
Gene Expression Profiling
Introns
Male
MicroRNAs
Models, Genetic
Molecular Sequence Data
Multigene Family
Petromyzon
Phylogeny
Protein Isoforms
Protein Subunits
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Issue Date: 2014
Citation: Nah G.S.S., Tay B.-H., Brenner S., Osato M., Venkatesh B. (2014). Characterization of the runx gene family in a jawless vertebrate, the Japanese lamprey (lethenteron japonicum). PLoS ONE 9 (11) : e113445. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0113445
Abstract: The cyclostomes (jawless vertebrates), comprising lampreys and hagfishes, are the sister group of jawed vertebrates (gnathostomes) and are hence an important group for the study of vertebrate evolution. In mammals, three Runx genes, Runx1, Runx2 and Runx3, encode transcription factors that are essential for cell proliferation and differentiation in major developmental pathways such as haematopoiesis, skeletogenesis and neurogenesis and are frequently associated with diseases. We describe here the characterization of Runx gene family members from a cyclostome, the Japanese lamprey (Lethenteron japonicum). The Japanese lamprey contains three Runx genes, RunxA, RunxB, and RunxC. However, phylogenetic and synteny analyses suggest that they are not one-to-one orthologs of gnathostome Runx1, Runx2 and Runx3. The major protein domains and motifs found in gnathostome Runx proteins are highly conserved in the lamprey Runx proteins. Although all gnathostome Runx genes each contain two alternative promoters, P1 (distal) and P2 (proximal), only lamprey RunxB possesses the alternative promoters; lamprey RunxA and RunxC contain only P2 and P1 promoter, respectively. Furthermore, the three lamprey Runx genes give rise to fewer alternative isoforms than the three gnathostome Runx genes. The promoters of the lamprey Runx genes lack the tandem Runx-binding motifs that are highly conserved among the P1 promoters of gnathostome Runx1, Runx2 and Runx3 genes; instead these promoters contain dispersed single Runx-binding motifs. The 39UTR of lamprey RunxB contains binding sites for miR-27 and miR-130b/301ab, which are conserved in mammalian Runx1 and Runx3, respectively. Overall, the Runx genes in lamprey seem to have experienced a different evolutionary trajectory from that of gnathostome Runx genes which are highly conserved all the way from cartilaginous fishes to mammals. © 2014 Nah et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161758
ISSN: 19326203
DOI: 10.1371/journal.pone.0113445
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