Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0113445
DC Field | Value | |
---|---|---|
dc.title | Characterization of the runx gene family in a jawless vertebrate, the Japanese lamprey (lethenteron japonicum) | |
dc.contributor.author | Nah G.S.S. | |
dc.contributor.author | Tay B.-H. | |
dc.contributor.author | Brenner S. | |
dc.contributor.author | Osato M. | |
dc.contributor.author | Venkatesh B. | |
dc.date.accessioned | 2019-11-07T05:04:37Z | |
dc.date.available | 2019-11-07T05:04:37Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Nah G.S.S., Tay B.-H., Brenner S., Osato M., Venkatesh B. (2014). Characterization of the runx gene family in a jawless vertebrate, the Japanese lamprey (lethenteron japonicum). PLoS ONE 9 (11) : e113445. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0113445 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161758 | |
dc.description.abstract | The cyclostomes (jawless vertebrates), comprising lampreys and hagfishes, are the sister group of jawed vertebrates (gnathostomes) and are hence an important group for the study of vertebrate evolution. In mammals, three Runx genes, Runx1, Runx2 and Runx3, encode transcription factors that are essential for cell proliferation and differentiation in major developmental pathways such as haematopoiesis, skeletogenesis and neurogenesis and are frequently associated with diseases. We describe here the characterization of Runx gene family members from a cyclostome, the Japanese lamprey (Lethenteron japonicum). The Japanese lamprey contains three Runx genes, RunxA, RunxB, and RunxC. However, phylogenetic and synteny analyses suggest that they are not one-to-one orthologs of gnathostome Runx1, Runx2 and Runx3. The major protein domains and motifs found in gnathostome Runx proteins are highly conserved in the lamprey Runx proteins. Although all gnathostome Runx genes each contain two alternative promoters, P1 (distal) and P2 (proximal), only lamprey RunxB possesses the alternative promoters; lamprey RunxA and RunxC contain only P2 and P1 promoter, respectively. Furthermore, the three lamprey Runx genes give rise to fewer alternative isoforms than the three gnathostome Runx genes. The promoters of the lamprey Runx genes lack the tandem Runx-binding motifs that are highly conserved among the P1 promoters of gnathostome Runx1, Runx2 and Runx3 genes; instead these promoters contain dispersed single Runx-binding motifs. The 39UTR of lamprey RunxB contains binding sites for miR-27 and miR-130b/301ab, which are conserved in mammalian Runx1 and Runx3, respectively. Overall, the Runx genes in lamprey seem to have experienced a different evolutionary trajectory from that of gnathostome Runx genes which are highly conserved all the way from cartilaginous fishes to mammals. © 2014 Nah et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | complementary DNA | |
dc.subject | microRNA | |
dc.subject | microRNA 130b | |
dc.subject | microRNA 131ab | |
dc.subject | microRNA 27 | |
dc.subject | transcription factor RUNX | |
dc.subject | transcription factor RUNX1 | |
dc.subject | transcription factor RUNX2 | |
dc.subject | transcription factor RUNX3 | |
dc.subject | unclassified drug | |
dc.subject | 3' untranslated region | |
dc.subject | fish protein | |
dc.subject | isoprotein | |
dc.subject | microRNA | |
dc.subject | protein subunit | |
dc.subject | transcription factor RUNX | |
dc.subject | 3' untranslated region | |
dc.subject | Article | |
dc.subject | binding site | |
dc.subject | cyclostome | |
dc.subject | gene | |
dc.subject | gene amplification | |
dc.subject | gene expression profiling | |
dc.subject | genetic organization | |
dc.subject | genetic trait | |
dc.subject | genome | |
dc.subject | lamprey | |
dc.subject | molecular cloning | |
dc.subject | molecular evolution | |
dc.subject | nonhuman | |
dc.subject | nucleotide sequence | |
dc.subject | orthology | |
dc.subject | phylogeny | |
dc.subject | promoter region | |
dc.subject | protein domain | |
dc.subject | protein motif | |
dc.subject | reverse transcription polymerase chain reaction | |
dc.subject | RunxA gene | |
dc.subject | RunxB gene | |
dc.subject | RunxC gene | |
dc.subject | sequence analysis | |
dc.subject | synteny | |
dc.subject | alternative RNA splicing | |
dc.subject | amino acid sequence | |
dc.subject | animal | |
dc.subject | biological model | |
dc.subject | classification | |
dc.subject | DNA sequence | |
dc.subject | exon | |
dc.subject | female | |
dc.subject | genetics | |
dc.subject | intron | |
dc.subject | male | |
dc.subject | metabolism | |
dc.subject | molecular genetics | |
dc.subject | multigene family | |
dc.subject | Petromyzon | |
dc.subject | protein subunit | |
dc.subject | sequence homology | |
dc.subject | Lethenteron japonicum | |
dc.subject | Vertebrata | |
dc.subject | 3' Untranslated Regions | |
dc.subject | Alternative Splicing | |
dc.subject | Amino Acid Sequence | |
dc.subject | Animals | |
dc.subject | Binding Sites | |
dc.subject | Core Binding Factor alpha Subunits | |
dc.subject | Evolution, Molecular | |
dc.subject | Exons | |
dc.subject | Female | |
dc.subject | Fish Proteins | |
dc.subject | Gene Expression Profiling | |
dc.subject | Introns | |
dc.subject | Male | |
dc.subject | MicroRNAs | |
dc.subject | Models, Genetic | |
dc.subject | Molecular Sequence Data | |
dc.subject | Multigene Family | |
dc.subject | Petromyzon | |
dc.subject | Phylogeny | |
dc.subject | Protein Isoforms | |
dc.subject | Protein Subunits | |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | |
dc.subject | Sequence Analysis, DNA | |
dc.subject | Sequence Homology, Amino Acid | |
dc.type | Article | |
dc.contributor.department | CANCER SCIENCE INSTITUTE OF SINGAPORE | |
dc.contributor.department | MEDICINE | |
dc.contributor.department | PAEDIATRICS | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.contributor.department | NUSHS PROJECT | |
dc.description.doi | 10.1371/journal.pone.0113445 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 9 | |
dc.description.issue | 11 | |
dc.description.page | e113445 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
Show simple item record
Files in This Item:
This item is licensed under a Creative Commons License