Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0113445
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dc.titleCharacterization of the runx gene family in a jawless vertebrate, the Japanese lamprey (lethenteron japonicum)
dc.contributor.authorNah G.S.S.
dc.contributor.authorTay B.-H.
dc.contributor.authorBrenner S.
dc.contributor.authorOsato M.
dc.contributor.authorVenkatesh B.
dc.date.accessioned2019-11-07T05:04:37Z
dc.date.available2019-11-07T05:04:37Z
dc.date.issued2014
dc.identifier.citationNah G.S.S., Tay B.-H., Brenner S., Osato M., Venkatesh B. (2014). Characterization of the runx gene family in a jawless vertebrate, the Japanese lamprey (lethenteron japonicum). PLoS ONE 9 (11) : e113445. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0113445
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161758
dc.description.abstractThe cyclostomes (jawless vertebrates), comprising lampreys and hagfishes, are the sister group of jawed vertebrates (gnathostomes) and are hence an important group for the study of vertebrate evolution. In mammals, three Runx genes, Runx1, Runx2 and Runx3, encode transcription factors that are essential for cell proliferation and differentiation in major developmental pathways such as haematopoiesis, skeletogenesis and neurogenesis and are frequently associated with diseases. We describe here the characterization of Runx gene family members from a cyclostome, the Japanese lamprey (Lethenteron japonicum). The Japanese lamprey contains three Runx genes, RunxA, RunxB, and RunxC. However, phylogenetic and synteny analyses suggest that they are not one-to-one orthologs of gnathostome Runx1, Runx2 and Runx3. The major protein domains and motifs found in gnathostome Runx proteins are highly conserved in the lamprey Runx proteins. Although all gnathostome Runx genes each contain two alternative promoters, P1 (distal) and P2 (proximal), only lamprey RunxB possesses the alternative promoters; lamprey RunxA and RunxC contain only P2 and P1 promoter, respectively. Furthermore, the three lamprey Runx genes give rise to fewer alternative isoforms than the three gnathostome Runx genes. The promoters of the lamprey Runx genes lack the tandem Runx-binding motifs that are highly conserved among the P1 promoters of gnathostome Runx1, Runx2 and Runx3 genes; instead these promoters contain dispersed single Runx-binding motifs. The 39UTR of lamprey RunxB contains binding sites for miR-27 and miR-130b/301ab, which are conserved in mammalian Runx1 and Runx3, respectively. Overall, the Runx genes in lamprey seem to have experienced a different evolutionary trajectory from that of gnathostome Runx genes which are highly conserved all the way from cartilaginous fishes to mammals. © 2014 Nah et al.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20191101
dc.subjectcomplementary DNA
dc.subjectmicroRNA
dc.subjectmicroRNA 130b
dc.subjectmicroRNA 131ab
dc.subjectmicroRNA 27
dc.subjecttranscription factor RUNX
dc.subjecttranscription factor RUNX1
dc.subjecttranscription factor RUNX2
dc.subjecttranscription factor RUNX3
dc.subjectunclassified drug
dc.subject3' untranslated region
dc.subjectfish protein
dc.subjectisoprotein
dc.subjectmicroRNA
dc.subjectprotein subunit
dc.subjecttranscription factor RUNX
dc.subject3' untranslated region
dc.subjectArticle
dc.subjectbinding site
dc.subjectcyclostome
dc.subjectgene
dc.subjectgene amplification
dc.subjectgene expression profiling
dc.subjectgenetic organization
dc.subjectgenetic trait
dc.subjectgenome
dc.subjectlamprey
dc.subjectmolecular cloning
dc.subjectmolecular evolution
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectorthology
dc.subjectphylogeny
dc.subjectpromoter region
dc.subjectprotein domain
dc.subjectprotein motif
dc.subjectreverse transcription polymerase chain reaction
dc.subjectRunxA gene
dc.subjectRunxB gene
dc.subjectRunxC gene
dc.subjectsequence analysis
dc.subjectsynteny
dc.subjectalternative RNA splicing
dc.subjectamino acid sequence
dc.subjectanimal
dc.subjectbiological model
dc.subjectclassification
dc.subjectDNA sequence
dc.subjectexon
dc.subjectfemale
dc.subjectgenetics
dc.subjectintron
dc.subjectmale
dc.subjectmetabolism
dc.subjectmolecular genetics
dc.subjectmultigene family
dc.subjectPetromyzon
dc.subjectprotein subunit
dc.subjectsequence homology
dc.subjectLethenteron japonicum
dc.subjectVertebrata
dc.subject3' Untranslated Regions
dc.subjectAlternative Splicing
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBinding Sites
dc.subjectCore Binding Factor alpha Subunits
dc.subjectEvolution, Molecular
dc.subjectExons
dc.subjectFemale
dc.subjectFish Proteins
dc.subjectGene Expression Profiling
dc.subjectIntrons
dc.subjectMale
dc.subjectMicroRNAs
dc.subjectModels, Genetic
dc.subjectMolecular Sequence Data
dc.subjectMultigene Family
dc.subjectPetromyzon
dc.subjectPhylogeny
dc.subjectProtein Isoforms
dc.subjectProtein Subunits
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSequence Analysis, DNA
dc.subjectSequence Homology, Amino Acid
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.contributor.departmentMEDICINE
dc.contributor.departmentPAEDIATRICS
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.contributor.departmentNUSHS PROJECT
dc.description.doi10.1371/journal.pone.0113445
dc.description.sourcetitlePLoS ONE
dc.description.volume9
dc.description.issue11
dc.description.pagee113445
dc.published.statePublished
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