Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pbio.0040003
Title: RNA viral community in human feces: Prevalence of plant pathogenic viruses
Authors: Zhang T.
Breitbart M.
Lee W.H.
Run J.-Q.
Wei C.L.
Soh S.W.L. 
Hibberd M.L. 
Liu E.T. 
Rohwer F.
Ruan Y.
Keywords: complementary DNA
virus RNA
adult
article
controlled study
DNA library
DNA sequence
feces analysis
feces microflora
food analysis
genome analysis
host pathogen interaction
human
molecular cloning
nonhuman
normal human
nucleotide sequence
pepper
pepper mildmottle virus
plant virus
population abundance
prevalence
RNA extraction
RNA virus
sequence analysis
virion
virus genome
virus identification
virus isolation
feces
food
infant
isolation and purification
molecular genetics
plant virus
reverse transcription polymerase chain reaction
virology
Pepper mild mottle virus
RNA viruses
Adult
Capsicum
Feces
Food
Humans
Infant
Molecular Sequence Data
Plant Viruses
Reverse Transcriptase Polymerase Chain Reaction
RNA Viruses
Issue Date: 2006
Citation: Zhang T., Breitbart M., Lee W.H., Run J.-Q., Wei C.L., Soh S.W.L., Hibberd M.L., Liu E.T., Rohwer F., Ruan Y. (2006). RNA viral community in human feces: Prevalence of plant pathogenic viruses. PLoS Biology 4 (1) : 108-118. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pbio.0040003
Abstract: The human gut is known to be a reservoir of a wide variety of microbes, including viruses. Many RNA viruses are known to be associated with gastroenteritis; however, the enteric RNA viral community present in healthy humans has not been described. Here, we present a comparative metagenomic analysis of the RNA viruses found in three fecal samples from two healthy human individuals. For this study, uncultured viruses were concentrated by tangential flow filtration, and viral RNA was extracted and cloned into shotgun viral cDNA libraries for sequencing analysis. The vast majority of the 36,769 viral sequences obtained were similar to plant pathogenic RNA viruses. The most abundant fecal virus in this study was pepper mild mottle virus (PMMV), which was found in high concentrations - up to 109 virions per gram of dry weight fecal matter. PMMV was also detected in 12 (66.7%) of 18 fecal samples collected from healthy individuals on two continents, indicating that this plant virus is prevalent in the human population. A number of pepper-based foods tested positive for PMMV, suggesting dietary origins for this virus. Intriguingly, the fecal PMMV was infectious to host plants, suggesting that humans might act as a vehicle for the dissemination of certain plant viruses. © 2006 Zhang et al.
Source Title: PLoS Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/161690
ISSN: 15457885
DOI: 10.1371/journal.pbio.0040003
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