Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pbio.0040003
DC Field | Value | |
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dc.title | RNA viral community in human feces: Prevalence of plant pathogenic viruses | |
dc.contributor.author | Zhang T. | |
dc.contributor.author | Breitbart M. | |
dc.contributor.author | Lee W.H. | |
dc.contributor.author | Run J.-Q. | |
dc.contributor.author | Wei C.L. | |
dc.contributor.author | Soh S.W.L. | |
dc.contributor.author | Hibberd M.L. | |
dc.contributor.author | Liu E.T. | |
dc.contributor.author | Rohwer F. | |
dc.contributor.author | Ruan Y. | |
dc.date.accessioned | 2019-11-06T09:38:39Z | |
dc.date.available | 2019-11-06T09:38:39Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Zhang T., Breitbart M., Lee W.H., Run J.-Q., Wei C.L., Soh S.W.L., Hibberd M.L., Liu E.T., Rohwer F., Ruan Y. (2006). RNA viral community in human feces: Prevalence of plant pathogenic viruses. PLoS Biology 4 (1) : 108-118. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pbio.0040003 | |
dc.identifier.issn | 15457885 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161690 | |
dc.description.abstract | The human gut is known to be a reservoir of a wide variety of microbes, including viruses. Many RNA viruses are known to be associated with gastroenteritis; however, the enteric RNA viral community present in healthy humans has not been described. Here, we present a comparative metagenomic analysis of the RNA viruses found in three fecal samples from two healthy human individuals. For this study, uncultured viruses were concentrated by tangential flow filtration, and viral RNA was extracted and cloned into shotgun viral cDNA libraries for sequencing analysis. The vast majority of the 36,769 viral sequences obtained were similar to plant pathogenic RNA viruses. The most abundant fecal virus in this study was pepper mild mottle virus (PMMV), which was found in high concentrations - up to 109 virions per gram of dry weight fecal matter. PMMV was also detected in 12 (66.7%) of 18 fecal samples collected from healthy individuals on two continents, indicating that this plant virus is prevalent in the human population. A number of pepper-based foods tested positive for PMMV, suggesting dietary origins for this virus. Intriguingly, the fecal PMMV was infectious to host plants, suggesting that humans might act as a vehicle for the dissemination of certain plant viruses. © 2006 Zhang et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | complementary DNA | |
dc.subject | virus RNA | |
dc.subject | adult | |
dc.subject | article | |
dc.subject | controlled study | |
dc.subject | DNA library | |
dc.subject | DNA sequence | |
dc.subject | feces analysis | |
dc.subject | feces microflora | |
dc.subject | food analysis | |
dc.subject | genome analysis | |
dc.subject | host pathogen interaction | |
dc.subject | human | |
dc.subject | molecular cloning | |
dc.subject | nonhuman | |
dc.subject | normal human | |
dc.subject | nucleotide sequence | |
dc.subject | pepper | |
dc.subject | pepper mildmottle virus | |
dc.subject | plant virus | |
dc.subject | population abundance | |
dc.subject | prevalence | |
dc.subject | RNA extraction | |
dc.subject | RNA virus | |
dc.subject | sequence analysis | |
dc.subject | virion | |
dc.subject | virus genome | |
dc.subject | virus identification | |
dc.subject | virus isolation | |
dc.subject | feces | |
dc.subject | food | |
dc.subject | infant | |
dc.subject | isolation and purification | |
dc.subject | molecular genetics | |
dc.subject | plant virus | |
dc.subject | reverse transcription polymerase chain reaction | |
dc.subject | virology | |
dc.subject | Pepper mild mottle virus | |
dc.subject | RNA viruses | |
dc.subject | Adult | |
dc.subject | Capsicum | |
dc.subject | Feces | |
dc.subject | Food | |
dc.subject | Humans | |
dc.subject | Infant | |
dc.subject | Molecular Sequence Data | |
dc.subject | Plant Viruses | |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | |
dc.subject | RNA Viruses | |
dc.type | Article | |
dc.contributor.department | PAEDIATRICS | |
dc.contributor.department | SAW SWEE HOCK SCHOOL OF PUBLIC HEALTH | |
dc.contributor.department | DEPT OF MEDICINE | |
dc.description.doi | 10.1371/journal.pbio.0040003 | |
dc.description.sourcetitle | PLoS Biology | |
dc.description.volume | 4 | |
dc.description.issue | 1 | |
dc.description.page | 108-118 | |
Appears in Collections: | Elements Staff Publications |
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