Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pbio.0040003
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dc.titleRNA viral community in human feces: Prevalence of plant pathogenic viruses
dc.contributor.authorZhang T.
dc.contributor.authorBreitbart M.
dc.contributor.authorLee W.H.
dc.contributor.authorRun J.-Q.
dc.contributor.authorWei C.L.
dc.contributor.authorSoh S.W.L.
dc.contributor.authorHibberd M.L.
dc.contributor.authorLiu E.T.
dc.contributor.authorRohwer F.
dc.contributor.authorRuan Y.
dc.date.accessioned2019-11-06T09:38:39Z
dc.date.available2019-11-06T09:38:39Z
dc.date.issued2006
dc.identifier.citationZhang T., Breitbart M., Lee W.H., Run J.-Q., Wei C.L., Soh S.W.L., Hibberd M.L., Liu E.T., Rohwer F., Ruan Y. (2006). RNA viral community in human feces: Prevalence of plant pathogenic viruses. PLoS Biology 4 (1) : 108-118. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pbio.0040003
dc.identifier.issn15457885
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161690
dc.description.abstractThe human gut is known to be a reservoir of a wide variety of microbes, including viruses. Many RNA viruses are known to be associated with gastroenteritis; however, the enteric RNA viral community present in healthy humans has not been described. Here, we present a comparative metagenomic analysis of the RNA viruses found in three fecal samples from two healthy human individuals. For this study, uncultured viruses were concentrated by tangential flow filtration, and viral RNA was extracted and cloned into shotgun viral cDNA libraries for sequencing analysis. The vast majority of the 36,769 viral sequences obtained were similar to plant pathogenic RNA viruses. The most abundant fecal virus in this study was pepper mild mottle virus (PMMV), which was found in high concentrations - up to 109 virions per gram of dry weight fecal matter. PMMV was also detected in 12 (66.7%) of 18 fecal samples collected from healthy individuals on two continents, indicating that this plant virus is prevalent in the human population. A number of pepper-based foods tested positive for PMMV, suggesting dietary origins for this virus. Intriguingly, the fecal PMMV was infectious to host plants, suggesting that humans might act as a vehicle for the dissemination of certain plant viruses. © 2006 Zhang et al.
dc.sourceUnpaywall 20191101
dc.subjectcomplementary DNA
dc.subjectvirus RNA
dc.subjectadult
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectDNA library
dc.subjectDNA sequence
dc.subjectfeces analysis
dc.subjectfeces microflora
dc.subjectfood analysis
dc.subjectgenome analysis
dc.subjecthost pathogen interaction
dc.subjecthuman
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectnormal human
dc.subjectnucleotide sequence
dc.subjectpepper
dc.subjectpepper mildmottle virus
dc.subjectplant virus
dc.subjectpopulation abundance
dc.subjectprevalence
dc.subjectRNA extraction
dc.subjectRNA virus
dc.subjectsequence analysis
dc.subjectvirion
dc.subjectvirus genome
dc.subjectvirus identification
dc.subjectvirus isolation
dc.subjectfeces
dc.subjectfood
dc.subjectinfant
dc.subjectisolation and purification
dc.subjectmolecular genetics
dc.subjectplant virus
dc.subjectreverse transcription polymerase chain reaction
dc.subjectvirology
dc.subjectPepper mild mottle virus
dc.subjectRNA viruses
dc.subjectAdult
dc.subjectCapsicum
dc.subjectFeces
dc.subjectFood
dc.subjectHumans
dc.subjectInfant
dc.subjectMolecular Sequence Data
dc.subjectPlant Viruses
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA Viruses
dc.typeArticle
dc.contributor.departmentDEPT OF PAEDIATRICS
dc.contributor.departmentSAW SWEE HOCK SCHOOL OF PUBLIC HEALTH
dc.contributor.departmentDEPT OF MEDICINE
dc.description.doi10.1371/journal.pbio.0040003
dc.description.sourcetitlePLoS Biology
dc.description.volume4
dc.description.issue1
dc.description.page108-118
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