Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0141161
Title: | Differences in expression level of Helios and neuropilin-1 do not distinguish thymus-derived from extrathymically-induced CD4+Foxp3+ regulatory T cells | Authors: | Szurek E. Cebula A. Wojciech L. Pietrzak M. Rempala G. Kisielow P. Ignatowicz L. |
Keywords: | Helios transcription factor Ikaros transcription factor neuropilin 1 unclassified drug DNA binding protein forkhead transcription factor Foxp3 protein, mouse neuropilin 1 transcription factor Zfpn1a2 protein, mouse animal cell animal experiment Article binding affinity CD4+ T lymphocyte controlled study lymphocyte activation lymphocyte differentiation microflora mouse nonhuman phenotype protein binding protein expression protein function protein localization regulatory T lymphocyte thymocyte animal C57BL mouse cell clone cell differentiation cell lineage cross breeding cytology female flow cytometry gene expression regulation genetics immunology immunophenotyping male thymus transgenic mouse Animals Cell Differentiation Cell Lineage Clone Cells Crosses, Genetic DNA-Binding Proteins Female Flow Cytometry Forkhead Transcription Factors Gene Expression Regulation Immunophenotyping Lymphocyte Activation Male Mice Mice, Inbred C57BL Mice, Transgenic Neuropilin-1 T-Lymphocytes, Regulatory Thymocytes Thymus Gland Transcription Factors |
Issue Date: | 2015 | Citation: | Szurek E., Cebula A., Wojciech L., Pietrzak M., Rempala G., Kisielow P., Ignatowicz L. (2015). Differences in expression level of Helios and neuropilin-1 do not distinguish thymus-derived from extrathymically-induced CD4+Foxp3+ regulatory T cells. PLoS ONE 10 (10) : e0141161. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0141161 | Rights: | Attribution 4.0 International | Abstract: | Helios transcription factor and semaphorin receptor Nrp-1 were originally described as constitutively expressed at high levels on CD4+Foxp3+ T regulatory cells of intrathymic origin (tTregs). On the other hand, CD4+Foxp3+ Tregs generated in the periphery (pTregs) or induced ex vivo (iTregs) were reported to express low levels of Helios and Nrp-1. Soon afterwards the reliability of Nrp-1 and Helios as markers discriminating between tTregs and pTregs was questioned and until now no consensus has been reached. Here, we used several genetically modified mouse strains that favor pTregs or tTregs formation and analyzed the TCR repertoire of these cells. We found that Tregs with variable levels of Nrp-1 and Helios were abundant in mice with compromised ability to support natural differentiation of tTregs or pTregs. We also report that TCR repertoires of Treg clones expressing high or low levels of Nrp-1 or Helios are similar and more alike repertoire of CD4+Foxp3+ than repertoire of CD4+Foxp3- thymocytes. These results show that high vs. low expression of Nrp-1 or Helios does not unequivocally identify Treg clones of thymic or peripheral origin. � 2015 Szurek et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161605 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0141161 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1371_journal_pone_0141161.pdf | 1.2 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License