Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0141161
DC Field | Value | |
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dc.title | Differences in expression level of Helios and neuropilin-1 do not distinguish thymus-derived from extrathymically-induced CD4+Foxp3+ regulatory T cells | |
dc.contributor.author | Szurek E. | |
dc.contributor.author | Cebula A. | |
dc.contributor.author | Wojciech L. | |
dc.contributor.author | Pietrzak M. | |
dc.contributor.author | Rempala G. | |
dc.contributor.author | Kisielow P. | |
dc.contributor.author | Ignatowicz L. | |
dc.date.accessioned | 2019-11-06T08:08:03Z | |
dc.date.available | 2019-11-06T08:08:03Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Szurek E., Cebula A., Wojciech L., Pietrzak M., Rempala G., Kisielow P., Ignatowicz L. (2015). Differences in expression level of Helios and neuropilin-1 do not distinguish thymus-derived from extrathymically-induced CD4+Foxp3+ regulatory T cells. PLoS ONE 10 (10) : e0141161. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0141161 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161605 | |
dc.description.abstract | Helios transcription factor and semaphorin receptor Nrp-1 were originally described as constitutively expressed at high levels on CD4+Foxp3+ T regulatory cells of intrathymic origin (tTregs). On the other hand, CD4+Foxp3+ Tregs generated in the periphery (pTregs) or induced ex vivo (iTregs) were reported to express low levels of Helios and Nrp-1. Soon afterwards the reliability of Nrp-1 and Helios as markers discriminating between tTregs and pTregs was questioned and until now no consensus has been reached. Here, we used several genetically modified mouse strains that favor pTregs or tTregs formation and analyzed the TCR repertoire of these cells. We found that Tregs with variable levels of Nrp-1 and Helios were abundant in mice with compromised ability to support natural differentiation of tTregs or pTregs. We also report that TCR repertoires of Treg clones expressing high or low levels of Nrp-1 or Helios are similar and more alike repertoire of CD4+Foxp3+ than repertoire of CD4+Foxp3- thymocytes. These results show that high vs. low expression of Nrp-1 or Helios does not unequivocally identify Treg clones of thymic or peripheral origin. � 2015 Szurek et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | Helios transcription factor | |
dc.subject | Ikaros transcription factor | |
dc.subject | neuropilin 1 | |
dc.subject | unclassified drug | |
dc.subject | DNA binding protein | |
dc.subject | forkhead transcription factor | |
dc.subject | Foxp3 protein, mouse | |
dc.subject | neuropilin 1 | |
dc.subject | transcription factor | |
dc.subject | Zfpn1a2 protein, mouse | |
dc.subject | animal cell | |
dc.subject | animal experiment | |
dc.subject | Article | |
dc.subject | binding affinity | |
dc.subject | CD4+ T lymphocyte | |
dc.subject | controlled study | |
dc.subject | lymphocyte activation | |
dc.subject | lymphocyte differentiation | |
dc.subject | microflora | |
dc.subject | mouse | |
dc.subject | nonhuman | |
dc.subject | phenotype | |
dc.subject | protein binding | |
dc.subject | protein expression | |
dc.subject | protein function | |
dc.subject | protein localization | |
dc.subject | regulatory T lymphocyte | |
dc.subject | thymocyte | |
dc.subject | animal | |
dc.subject | C57BL mouse | |
dc.subject | cell clone | |
dc.subject | cell differentiation | |
dc.subject | cell lineage | |
dc.subject | cross breeding | |
dc.subject | cytology | |
dc.subject | female | |
dc.subject | flow cytometry | |
dc.subject | gene expression regulation | |
dc.subject | genetics | |
dc.subject | immunology | |
dc.subject | immunophenotyping | |
dc.subject | male | |
dc.subject | thymus | |
dc.subject | transgenic mouse | |
dc.subject | Animals | |
dc.subject | Cell Differentiation | |
dc.subject | Cell Lineage | |
dc.subject | Clone Cells | |
dc.subject | Crosses, Genetic | |
dc.subject | DNA-Binding Proteins | |
dc.subject | Female | |
dc.subject | Flow Cytometry | |
dc.subject | Forkhead Transcription Factors | |
dc.subject | Gene Expression Regulation | |
dc.subject | Immunophenotyping | |
dc.subject | Lymphocyte Activation | |
dc.subject | Male | |
dc.subject | Mice | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Mice, Transgenic | |
dc.subject | Neuropilin-1 | |
dc.subject | T-Lymphocytes, Regulatory | |
dc.subject | Thymocytes | |
dc.subject | Thymus Gland | |
dc.subject | Transcription Factors | |
dc.type | Article | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.description.doi | 10.1371/journal.pone.0141161 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 10 | |
dc.description.issue | 10 | |
dc.description.page | e0141161 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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