Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0122634
Title: Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f. Sp. Cubense
Authors: Ding Z.
Li M.
Sun F.
Xi P.
Sun L.
Zhang L. 
Jiang Z.
Keywords: beauvericin
chitin
fusaric acid
mitogen activated protein kinase
mitogen activated protein kinase FoBck1
mitogen activated protein kinase FoMkk2
mitogen activated protein kinase FoSlt2
peroxidase
siderophore
unclassified drug
beauvericin
depsipeptide
fusaric acid
mitogen activated protein kinase
siderophore
Article
banana
biochemical analysis
bioinformatics
cell wall integrity
cellular parameters
computer model
conidium
controlled study
deletion mutant
enzyme activity
FoBck1 gene
FoMkk2 gene
FoSlt2 gene
fungal virulence
fungus hyphae
fungus mutant
Fusarium cubense
gene
gene amplification
gene control
gene mutation
genetic trait
nonhuman
null mutation
open reading frame
phenotype
sensitivity analysis
signal transduction
transcription regulation
biology
biosynthesis
cell wall
cytology
enzymology
Fusarium
genetic transcription
genetics
metabolism
mutation
pathogenicity
virulence
Fusarium
Fusarium oxysporum f. cubense
Cell Wall
Computational Biology
Depsipeptides
Fusaric Acid
Fusarium
Hyphae
Mitogen-Activated Protein Kinases
Mutation
Siderophores
Transcription, Genetic
Virulence
Issue Date: 2015
Citation: Ding Z., Li M., Sun F., Xi P., Sun L., Zhang L., Jiang Z. (2015). Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f. Sp. Cubense. PLoS ONE 10 (4) : e0122634. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0122634
Abstract: Fusarium oxysporum f. Sp. cubense(FOC) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. However, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. In this study, we showed that null mutation of three mitogen-activated protein (MAP) kinase genes, designated as FoSlt2, FoMkk2and FoBck1, respectively, led to substantial attenuation in fungal virulence on banana plants. Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid. Biochemical analysis further confirmed the essential role of MAP kinases in modulating the production of fusaric acid, which was a crucial phytotoxin in accelerating development of Fusarium wilt symptoms in banana plants. Additionally, we found that the MAP kinase FoSlt2 was required for siderophore biosynthesis under iron-depletion conditions. Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2. Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence. © 2015 Ding et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161522
ISSN: 19326203
DOI: 10.1371/journal.pone.0122634
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