Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0122634
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dc.titleMitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f. Sp. Cubense
dc.contributor.authorDing Z.
dc.contributor.authorLi M.
dc.contributor.authorSun F.
dc.contributor.authorXi P.
dc.contributor.authorSun L.
dc.contributor.authorZhang L.
dc.contributor.authorJiang Z.
dc.date.accessioned2019-11-06T01:32:44Z
dc.date.available2019-11-06T01:32:44Z
dc.date.issued2015
dc.identifier.citationDing Z., Li M., Sun F., Xi P., Sun L., Zhang L., Jiang Z. (2015). Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f. Sp. Cubense. PLoS ONE 10 (4) : e0122634. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0122634
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161522
dc.description.abstractFusarium oxysporum f. Sp. cubense(FOC) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. However, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. In this study, we showed that null mutation of three mitogen-activated protein (MAP) kinase genes, designated as FoSlt2, FoMkk2and FoBck1, respectively, led to substantial attenuation in fungal virulence on banana plants. Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid. Biochemical analysis further confirmed the essential role of MAP kinases in modulating the production of fusaric acid, which was a crucial phytotoxin in accelerating development of Fusarium wilt symptoms in banana plants. Additionally, we found that the MAP kinase FoSlt2 was required for siderophore biosynthesis under iron-depletion conditions. Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2. Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence. © 2015 Ding et al.
dc.sourceUnpaywall
dc.subjectbeauvericin
dc.subjectchitin
dc.subjectfusaric acid
dc.subjectmitogen activated protein kinase
dc.subjectmitogen activated protein kinase FoBck1
dc.subjectmitogen activated protein kinase FoMkk2
dc.subjectmitogen activated protein kinase FoSlt2
dc.subjectperoxidase
dc.subjectsiderophore
dc.subjectunclassified drug
dc.subjectbeauvericin
dc.subjectdepsipeptide
dc.subjectfusaric acid
dc.subjectmitogen activated protein kinase
dc.subjectsiderophore
dc.subjectArticle
dc.subjectbanana
dc.subjectbiochemical analysis
dc.subjectbioinformatics
dc.subjectcell wall integrity
dc.subjectcellular parameters
dc.subjectcomputer model
dc.subjectconidium
dc.subjectcontrolled study
dc.subjectdeletion mutant
dc.subjectenzyme activity
dc.subjectFoBck1 gene
dc.subjectFoMkk2 gene
dc.subjectFoSlt2 gene
dc.subjectfungal virulence
dc.subjectfungus hyphae
dc.subjectfungus mutant
dc.subjectFusarium cubense
dc.subjectgene
dc.subjectgene amplification
dc.subjectgene control
dc.subjectgene mutation
dc.subjectgenetic trait
dc.subjectnonhuman
dc.subjectnull mutation
dc.subjectopen reading frame
dc.subjectphenotype
dc.subjectsensitivity analysis
dc.subjectsignal transduction
dc.subjecttranscription regulation
dc.subjectbiology
dc.subjectbiosynthesis
dc.subjectcell wall
dc.subjectcytology
dc.subjectenzymology
dc.subjectFusarium
dc.subjectgenetic transcription
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmutation
dc.subjectpathogenicity
dc.subjectvirulence
dc.subjectFusarium
dc.subjectFusarium oxysporum f. cubense
dc.subjectCell Wall
dc.subjectComputational Biology
dc.subjectDepsipeptides
dc.subjectFusaric Acid
dc.subjectFusarium
dc.subjectHyphae
dc.subjectMitogen-Activated Protein Kinases
dc.subjectMutation
dc.subjectSiderophores
dc.subjectTranscription, Genetic
dc.subjectVirulence
dc.typeArticle
dc.contributor.departmentBIOLOGY (NU)
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.1371/journal.pone.0122634
dc.description.sourcetitlePLoS ONE
dc.description.volume10
dc.description.issue4
dc.description.pagee0122634
dc.published.statePublished
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