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https://doi.org/10.1371/journal.pone.0122403
| Title: | Genomic analysis of a mycobacterium bovisbacillus Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis | Authors: | Li X. Chen L. Zhu Y. Yu X. Cao J. Wang R. Lv X. He J. Guo A. Huang H. Zheng H. Liu S. |
Keywords: | amikacin aminosalicylic acid cycloserine epitope ethambutol ethionamide isoniazid kanamycin moxifloxacin ofloxacin rifabutin rifampicin streptomycin virulence factor epitope adult Article bacterial strain bacterial virulence bacterium identification bacterium isolation chromosome replication controlled study dnaA gene dnaN gene gene duplication genome analysis human lung tuberculosis male Mycobacterium bovis BCG next generation sequencing nonhuman pathogenesis bacterial gene case report chemistry DNA sequence genetics genomics high throughput sequencing isolation and purification microbiology molecular genetics Mycobacterium bovis Mycobacterium tuberculosis phylogeny Tuberculosis, Pulmonary virulence Mycobacterium Adult Epitopes Genes, Bacterial Genomics High-Throughput Nucleotide Sequencing Humans Male Molecular Sequence Data Mycobacterium bovis Mycobacterium tuberculosis Phylogeny Sequence Analysis, DNA Tuberculosis, Pulmonary Virulence |
Issue Date: | 2015 | Citation: | Li X., Chen L., Zhu Y., Yu X., Cao J., Wang R., Lv X., He J., Guo A., Huang H., Zheng H., Liu S. (2015). Genomic analysis of a mycobacterium bovisbacillus Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis. PLoS ONE 10 (4) : e0122403. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0122403 | Rights: | Attribution 4.0 International | Abstract: | For years, bacillus Calmette-Guérin (BCG) has served as the unique vaccine against tuberculosis and has generally been regarded as safe. However, a clinical strain labeled 3281 that was isolated from a TB patient was identified to be BCG. Via the combination of next-generation sequencing (NGS) and comparative genomic analysis, unique 3281 genetic characteristics were revealed. A region containing the dnaA and dnaN genes that is closely related to the initial chromosome replication was found to repeat three times on the BCG Pasteur-specific tandem duplication region DU1. Due to the minimum number of epitopes in BCG strains, 3281 was inferred to have a high possibility for immune evasion. Additionally, variations in the virulence genes and predictions for potential virulence factors were analyzed. Overall, we report a pathogen that has never previously been thought to be pathogenic and initial insights that are focused on the genetic characteristics of virulent BCG. © 2015 Li et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161519 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0122403 | Rights: | Attribution 4.0 International |
| Appears in Collections: | Staff Publications Elements |
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