Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0122403
Title: Genomic analysis of a mycobacterium bovisbacillus Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis
Authors: Li X.
Chen L.
Zhu Y.
Yu X. 
Cao J.
Wang R.
Lv X.
He J.
Guo A.
Huang H.
Zheng H.
Liu S.
Keywords: amikacin
aminosalicylic acid
cycloserine
epitope
ethambutol
ethionamide
isoniazid
kanamycin
moxifloxacin
ofloxacin
rifabutin
rifampicin
streptomycin
virulence factor
epitope
adult
Article
bacterial strain
bacterial virulence
bacterium identification
bacterium isolation
chromosome replication
controlled study
dnaA gene
dnaN gene
gene duplication
genome analysis
human
lung tuberculosis
male
Mycobacterium bovis BCG
next generation sequencing
nonhuman
pathogenesis
bacterial gene
case report
chemistry
DNA sequence
genetics
genomics
high throughput sequencing
isolation and purification
microbiology
molecular genetics
Mycobacterium bovis
Mycobacterium tuberculosis
phylogeny
Tuberculosis, Pulmonary
virulence
Mycobacterium
Adult
Epitopes
Genes, Bacterial
Genomics
High-Throughput Nucleotide Sequencing
Humans
Male
Molecular Sequence Data
Mycobacterium bovis
Mycobacterium tuberculosis
Phylogeny
Sequence Analysis, DNA
Tuberculosis, Pulmonary
Virulence
Issue Date: 2015
Citation: Li X., Chen L., Zhu Y., Yu X., Cao J., Wang R., Lv X., He J., Guo A., Huang H., Zheng H., Liu S. (2015). Genomic analysis of a mycobacterium bovisbacillus Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis. PLoS ONE 10 (4) : e0122403. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0122403
Rights: Attribution 4.0 International
Abstract: For years, bacillus Calmette-Guérin (BCG) has served as the unique vaccine against tuberculosis and has generally been regarded as safe. However, a clinical strain labeled 3281 that was isolated from a TB patient was identified to be BCG. Via the combination of next-generation sequencing (NGS) and comparative genomic analysis, unique 3281 genetic characteristics were revealed. A region containing the dnaA and dnaN genes that is closely related to the initial chromosome replication was found to repeat three times on the BCG Pasteur-specific tandem duplication region DU1. Due to the minimum number of epitopes in BCG strains, 3281 was inferred to have a high possibility for immune evasion. Additionally, variations in the virulence genes and predictions for potential virulence factors were analyzed. Overall, we report a pathogen that has never previously been thought to be pathogenic and initial insights that are focused on the genetic characteristics of virulent BCG. © 2015 Li et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161519
ISSN: 19326203
DOI: 10.1371/journal.pone.0122403
Rights: Attribution 4.0 International
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