1. ScholarBank@NUS
  2. 1. Staff
  3. Staff Publications
Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0122403
DC FieldValue
dc.titleGenomic analysis of a mycobacterium bovisbacillus Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis
dc.contributor.authorLi X.
dc.contributor.authorChen L.
dc.contributor.authorZhu Y.
dc.contributor.authorYu X.
dc.contributor.authorCao J.
dc.contributor.authorWang R.
dc.contributor.authorLv X.
dc.contributor.authorHe J.
dc.contributor.authorGuo A.
dc.contributor.authorHuang H.
dc.contributor.authorZheng H.
dc.contributor.authorLiu S.
dc.date.accessioned2019-11-06T01:32:13Z
dc.date.available2019-11-06T01:32:13Z
dc.date.issued2015
dc.identifier.citationLi X., Chen L., Zhu Y., Yu X., Cao J., Wang R., Lv X., He J., Guo A., Huang H., Zheng H., Liu S. (2015). Genomic analysis of a mycobacterium bovisbacillus Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis. PLoS ONE 10 (4) : e0122403. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0122403
dc.identifier.issn19326203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161519
dc.description.abstractFor years, bacillus Calmette-Guérin (BCG) has served as the unique vaccine against tuberculosis and has generally been regarded as safe. However, a clinical strain labeled 3281 that was isolated from a TB patient was identified to be BCG. Via the combination of next-generation sequencing (NGS) and comparative genomic analysis, unique 3281 genetic characteristics were revealed. A region containing the dnaA and dnaN genes that is closely related to the initial chromosome replication was found to repeat three times on the BCG Pasteur-specific tandem duplication region DU1. Due to the minimum number of epitopes in BCG strains, 3281 was inferred to have a high possibility for immune evasion. Additionally, variations in the virulence genes and predictions for potential virulence factors were analyzed. Overall, we report a pathogen that has never previously been thought to be pathogenic and initial insights that are focused on the genetic characteristics of virulent BCG. © 2015 Li et al.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20191101
dc.subjectamikacin
dc.subjectaminosalicylic acid
dc.subjectcycloserine
dc.subjectepitope
dc.subjectethambutol
dc.subjectethionamide
dc.subjectisoniazid
dc.subjectkanamycin
dc.subjectmoxifloxacin
dc.subjectofloxacin
dc.subjectrifabutin
dc.subjectrifampicin
dc.subjectstreptomycin
dc.subjectvirulence factor
dc.subjectepitope
dc.subjectadult
dc.subjectArticle
dc.subjectbacterial strain
dc.subjectbacterial virulence
dc.subjectbacterium identification
dc.subjectbacterium isolation
dc.subjectchromosome replication
dc.subjectcontrolled study
dc.subjectdnaA gene
dc.subjectdnaN gene
dc.subjectgene duplication
dc.subjectgenome analysis
dc.subjecthuman
dc.subjectlung tuberculosis
dc.subjectmale
dc.subjectMycobacterium bovis BCG
dc.subjectnext generation sequencing
dc.subjectnonhuman
dc.subjectpathogenesis
dc.subjectbacterial gene
dc.subjectcase report
dc.subjectchemistry
dc.subjectDNA sequence
dc.subjectgenetics
dc.subjectgenomics
dc.subjecthigh throughput sequencing
dc.subjectisolation and purification
dc.subjectmicrobiology
dc.subjectmolecular genetics
dc.subjectMycobacterium bovis
dc.subjectMycobacterium tuberculosis
dc.subjectphylogeny
dc.subjectTuberculosis, Pulmonary
dc.subjectvirulence
dc.subjectMycobacterium
dc.subjectAdult
dc.subjectEpitopes
dc.subjectGenes, Bacterial
dc.subjectGenomics
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.subjectHumans
dc.subjectMale
dc.subjectMolecular Sequence Data
dc.subjectMycobacterium bovis
dc.subjectMycobacterium tuberculosis
dc.subjectPhylogeny
dc.subjectSequence Analysis, DNA
dc.subjectTuberculosis, Pulmonary
dc.subjectVirulence
dc.typeArticle
dc.contributor.departmentELECTRICAL AND COMPUTER ENGINEERING
dc.description.doi10.1371/journal.pone.0122403
dc.description.sourcetitlePLoS ONE
dc.description.volume10
dc.description.issue4
dc.description.pagee0122403
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0122403.pdf1.75 MBAdobe PDF

OPEN

PublishedView/Download

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons