Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0125354
Title: Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element
Authors: Sato M. 
Kawano M.
Ohkura M.
Gengyo-Ando K.
Nakai J.
Hayashi Y.
Keywords: protein G CaMP7
red fluorescent protein
transactivator protein
unclassified drug
calcium
green fluorescent protein
membrane protein
tetracycline
adult
animal cell
animal tissue
Article
calcium cell level
cell population
connectome
controlled study
fluorescence imaging
hippocampal CA1 region
in vivo study
mouse
nonhuman
nucleic acid structure
protein expression
pyramidal nerve cell
signal detection
tetracycline response element
animal
diagnostic imaging
DNA responsive element
fluorescence microscopy
fluorescence resonance energy transfer
gene expression
genetics
metabolism
procedures
transgenic mouse
Mus
Mus musculus
Animals
CA1 Region, Hippocampal
Calcium
Diagnostic Imaging
Fluorescence Resonance Energy Transfer
Gene Expression
Green Fluorescent Proteins
Membrane Proteins
Mice
Mice, Transgenic
Microscopy, Fluorescence
Pyramidal Cells
Response Elements
Tetracycline
Issue Date: 2015
Citation: Sato M., Kawano M., Ohkura M., Gengyo-Ando K., Nakai J., Hayashi Y. (2015). Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element. PLoS ONE 10 (5) : e0125354. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0125354
Rights: Attribution 4.0 International
Abstract: The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo. © 2015 Sato et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161515
ISSN: 19326203
DOI: 10.1371/journal.pone.0125354
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications
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