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https://doi.org/10.1371/journal.pone.0125354
DC Field | Value | |
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dc.title | Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element | |
dc.contributor.author | Sato M. | |
dc.contributor.author | Kawano M. | |
dc.contributor.author | Ohkura M. | |
dc.contributor.author | Gengyo-Ando K. | |
dc.contributor.author | Nakai J. | |
dc.contributor.author | Hayashi Y. | |
dc.date.accessioned | 2019-11-06T01:31:33Z | |
dc.date.available | 2019-11-06T01:31:33Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Sato M., Kawano M., Ohkura M., Gengyo-Ando K., Nakai J., Hayashi Y. (2015). Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element. PLoS ONE 10 (5) : e0125354. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0125354 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/161515 | |
dc.description.abstract | The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo. © 2015 Sato et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20191101 | |
dc.subject | protein G CaMP7 | |
dc.subject | red fluorescent protein | |
dc.subject | transactivator protein | |
dc.subject | unclassified drug | |
dc.subject | calcium | |
dc.subject | green fluorescent protein | |
dc.subject | membrane protein | |
dc.subject | tetracycline | |
dc.subject | adult | |
dc.subject | animal cell | |
dc.subject | animal tissue | |
dc.subject | Article | |
dc.subject | calcium cell level | |
dc.subject | cell population | |
dc.subject | connectome | |
dc.subject | controlled study | |
dc.subject | fluorescence imaging | |
dc.subject | hippocampal CA1 region | |
dc.subject | in vivo study | |
dc.subject | mouse | |
dc.subject | nonhuman | |
dc.subject | nucleic acid structure | |
dc.subject | protein expression | |
dc.subject | pyramidal nerve cell | |
dc.subject | signal detection | |
dc.subject | tetracycline response element | |
dc.subject | animal | |
dc.subject | diagnostic imaging | |
dc.subject | DNA responsive element | |
dc.subject | fluorescence microscopy | |
dc.subject | fluorescence resonance energy transfer | |
dc.subject | gene expression | |
dc.subject | genetics | |
dc.subject | metabolism | |
dc.subject | procedures | |
dc.subject | transgenic mouse | |
dc.subject | Mus | |
dc.subject | Mus musculus | |
dc.subject | Animals | |
dc.subject | CA1 Region, Hippocampal | |
dc.subject | Calcium | |
dc.subject | Diagnostic Imaging | |
dc.subject | Fluorescence Resonance Energy Transfer | |
dc.subject | Gene Expression | |
dc.subject | Green Fluorescent Proteins | |
dc.subject | Membrane Proteins | |
dc.subject | Mice | |
dc.subject | Mice, Transgenic | |
dc.subject | Microscopy, Fluorescence | |
dc.subject | Pyramidal Cells | |
dc.subject | Response Elements | |
dc.subject | Tetracycline | |
dc.type | Article | |
dc.contributor.department | INTERACTIVE & DIGITAL MEDIA INSTITUTE | |
dc.description.doi | 10.1371/journal.pone.0125354 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 10 | |
dc.description.issue | 5 | |
dc.description.page | e0125354 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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10_1371_journal_pone_0125354.pdf | 9.59 MB | Adobe PDF | OPEN | Published | View/Download |
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