Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0125618
Title: High affinity antibodies against influenza characterize the plasmablast response in SLE patients after vaccination
Authors: Kaur K.
Zheng N.-Y.
Smith K. 
Huang M.
Li L.
Pauli N.T.
Henry Dunand C.J.
Lee J.-H.
Morrissey M.
Wu Y.
Joachims M.L.
Munroe M.E.
Lau D.
Qu X.
Krammer F.
Wrammert J.
Palese P.
Ahmed R.
James J.A.
Wilson P.C.
Keywords: influenza vaccine
monoclonal antibody
virus antibody
influenza vaccine
monoclonal antibody
antibody production
antibody response
antigen specificity
Article
B lymphocyte
binding affinity
clinical article
controlled study
drug response
germinal center
human
human cell
immune dysregulation
influenza vaccination
plasmablast
systemic lupus erythematosus
antibody affinity
case control study
immunology
Orthomyxoviridae
systemic lupus erythematosus
Antibodies, Monoclonal
Antibody Affinity
Antibody Formation
Case-Control Studies
Humans
Influenza Vaccines
Lupus Erythematosus, Systemic
Orthomyxoviridae
Issue Date: 2015
Citation: Kaur K., Zheng N.-Y., Smith K., Huang M., Li L., Pauli N.T., Henry Dunand C.J., Lee J.-H., Morrissey M., Wu Y., Joachims M.L., Munroe M.E., Lau D., Qu X., Krammer F., Wrammert J., Palese P., Ahmed R., James J.A., Wilson P.C. (2015). High affinity antibodies against influenza characterize the plasmablast response in SLE patients after vaccination. PLoS ONE 10 (5) : e0125618. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0125618
Rights: Attribution 4.0 International
Abstract: Breakdown of B cell tolerance is a cardinal feature of systemic lupus erythematosus (SLE). Increased numbers of autoreactive mature naïve B cells have been described in SLE patients and autoantibodies have been shown to arise from autoreactive and non-autoreactive precursors. How these defects, in the regulation of B cell tolerance and selection, influence germinal center (GC) reactions that are directed towards foreign antigens has yet to be investigated. Here, we examined the characteristics of post-GC foreign antigen-specific B cells from SLE patients and healthy controls by analyzing monoclonal antibodies generated from plasmablasts induced specifically by influenza vaccination. We report that many of the SLE patients had anti-influenza antibodies with higher binding affinity and neutralization capacity than those from controls. Although overall frequencies of autoreactivity in the influenza-specific plasmablasts were similar for SLE patients and controls, the variable gene repertoire of influenza-specific plasmablasts from SLE patients was altered, with increased usage of JH6 and long heavy chain CDR3 segments. We found that high affinity anti-influenza antibodies generally characterize the plasmablast responses of SLE patients with low levels of autoreactivity; however, certain exceptions were noted. The high-avidity antibody responses in SLE patients may also be correlated with cytokines that are abnormally expressed in lupus. These findings provide insights into the effects of dysregulated immunity on the quality of antibody responses following influenza vaccination and further our understanding of the underlying abnormalities of lupus. © 2015 Kaur et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161514
ISSN: 19326203
DOI: 10.1371/journal.pone.0125618
Rights: Attribution 4.0 International
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