Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0125618
Title: | High affinity antibodies against influenza characterize the plasmablast response in SLE patients after vaccination | Authors: | Kaur K. Zheng N.-Y. Smith K. Huang M. Li L. Pauli N.T. Henry Dunand C.J. Lee J.-H. Morrissey M. Wu Y. Joachims M.L. Munroe M.E. Lau D. Qu X. Krammer F. Wrammert J. Palese P. Ahmed R. James J.A. Wilson P.C. |
Keywords: | influenza vaccine monoclonal antibody virus antibody influenza vaccine monoclonal antibody antibody production antibody response antigen specificity Article B lymphocyte binding affinity clinical article controlled study drug response germinal center human human cell immune dysregulation influenza vaccination plasmablast systemic lupus erythematosus antibody affinity case control study immunology Orthomyxoviridae systemic lupus erythematosus Antibodies, Monoclonal Antibody Affinity Antibody Formation Case-Control Studies Humans Influenza Vaccines Lupus Erythematosus, Systemic Orthomyxoviridae |
Issue Date: | 2015 | Citation: | Kaur K., Zheng N.-Y., Smith K., Huang M., Li L., Pauli N.T., Henry Dunand C.J., Lee J.-H., Morrissey M., Wu Y., Joachims M.L., Munroe M.E., Lau D., Qu X., Krammer F., Wrammert J., Palese P., Ahmed R., James J.A., Wilson P.C. (2015). High affinity antibodies against influenza characterize the plasmablast response in SLE patients after vaccination. PLoS ONE 10 (5) : e0125618. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0125618 | Rights: | Attribution 4.0 International | Abstract: | Breakdown of B cell tolerance is a cardinal feature of systemic lupus erythematosus (SLE). Increased numbers of autoreactive mature naïve B cells have been described in SLE patients and autoantibodies have been shown to arise from autoreactive and non-autoreactive precursors. How these defects, in the regulation of B cell tolerance and selection, influence germinal center (GC) reactions that are directed towards foreign antigens has yet to be investigated. Here, we examined the characteristics of post-GC foreign antigen-specific B cells from SLE patients and healthy controls by analyzing monoclonal antibodies generated from plasmablasts induced specifically by influenza vaccination. We report that many of the SLE patients had anti-influenza antibodies with higher binding affinity and neutralization capacity than those from controls. Although overall frequencies of autoreactivity in the influenza-specific plasmablasts were similar for SLE patients and controls, the variable gene repertoire of influenza-specific plasmablasts from SLE patients was altered, with increased usage of JH6 and long heavy chain CDR3 segments. We found that high affinity anti-influenza antibodies generally characterize the plasmablast responses of SLE patients with low levels of autoreactivity; however, certain exceptions were noted. The high-avidity antibody responses in SLE patients may also be correlated with cytokines that are abnormally expressed in lupus. These findings provide insights into the effects of dysregulated immunity on the quality of antibody responses following influenza vaccination and further our understanding of the underlying abnormalities of lupus. © 2015 Kaur et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161514 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0125618 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1371_journal_pone_0125618.pdf | 1.85 MB | Adobe PDF | OPEN | Published | View/Download |
This item is licensed under a Creative Commons License