Please use this identifier to cite or link to this item:
Title: Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma
Authors: Kanojia D. 
Garg M.
Saini S.
Agarwal S.
Parashar D.
Jagadish N.
Seth A.
Bhatnagar A.
Gupta A.
Kumar R.
Lohiya N.K.
Suri A.
Keywords: cancer testis antigen
cyclin B
cyclin D
cyclin dependent kinase 1
cyclin dependent kinase 4
cyclin E
protein p16
protein p21
sperm associated antigen 9
unclassified drug
antigen expression
cancer cell
cancer grading
cell cycle arrest
cell cycle G0 phase
cell cycle G1 phase
cell invasion
cell migration
cell proliferation
controlled study
down regulation
gene expression regulation
gene silencing
genetic association
human cell
human tissue
humoral immunity
major clinical study
middle aged
non muscle invasive bladder cancer
transitional cell carcinoma
Adaptor Proteins, Signal Transducing
Carcinoma, Transitional Cell
Cell Cycle
Cell Line, Tumor
Cell Proliferation
Middle Aged
Tumor Markers, Biological
Urinary Bladder Neoplasms
Issue Date: 2013
Citation: Kanojia D., Garg M., Saini S., Agarwal S., Parashar D., Jagadish N., Seth A., Bhatnagar A., Gupta A., Kumar R., Lohiya N.K., Suri A. (2013). Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma. PLoS ONE 8 (12) : e81348. ScholarBank@NUS Repository.
Abstract: Background: Majority of bladder cancer deaths are caused due to transitional cell carcinoma (TCC) which is the most prevalent and chemoresistant malignancy of urinary bladder. Therefore, we analyzed the role of Sperm associated antigen 9 (SPAG9) in bladder TCC. Methodology and Findings: We examined SPAG9 expression and humoral response in 125 bladder TCC patients. Four bladder cancer cell lines were assessed for SPAG9 expression. In addition, we investigated the effect of SPAG9 ablation on cellular proliferation, cell cycle, migration and invasion in UM-UC-3 bladder cancer cells by employing gene silencing approach. Our SPAG9 gene and protein expression analysis revealed SPAG9 expression in 81% of bladder TCC tissue specimens. High SPAG9 expression (>60% SPAG9 positive cells) was found to be significantly associated with superficial non-muscle invasive stage (P = 0.042) and low grade tumors (P = 0.002) suggesting SPAG9 putative role in early spread and tumorigenesis. Humoral response against SPAG9 was observed in 95% of patients found positive for SPAG9 expression. All four bladder cancer cell lines revealed SPAG9 expression. In addition, SPAG9 gene silencing in UM-UC-3 cells resulted in induction of G 0 -G 1 arrest characterized by up-regulation of p16 and p21 and consequent down-regulation of cyclin E, cyclin D and cyclin B, CDK4 and CDK1. Further, SPAG9 gene silencing also resulted in reduction in cellular growth, and migration and invasion ability of cancer cells in vitro. Conclusions: Collectively, our data in clinical specimens indicated that SPAG9 is potential biomarker and therapeutic target for bladder TCC. © 2013 Kanojia et al.
Source Title: PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0081348
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0081348.pdf24.11 MBAdobe PDF




checked on Jul 5, 2020

Page view(s)

checked on Jul 2, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.