Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0081348
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dc.titleSperm associated antigen 9 plays an important role in bladder transitional cell carcinoma
dc.contributor.authorKanojia D.
dc.contributor.authorGarg M.
dc.contributor.authorSaini S.
dc.contributor.authorAgarwal S.
dc.contributor.authorParashar D.
dc.contributor.authorJagadish N.
dc.contributor.authorSeth A.
dc.contributor.authorBhatnagar A.
dc.contributor.authorGupta A.
dc.contributor.authorKumar R.
dc.contributor.authorLohiya N.K.
dc.contributor.authorSuri A.
dc.date.accessioned2019-11-05T02:07:37Z
dc.date.available2019-11-05T02:07:37Z
dc.date.issued2013
dc.identifier.citationKanojia D., Garg M., Saini S., Agarwal S., Parashar D., Jagadish N., Seth A., Bhatnagar A., Gupta A., Kumar R., Lohiya N.K., Suri A. (2013). Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma. PLoS ONE 8 (12) : e81348. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0081348
dc.identifier.issn1932-6203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/161446
dc.description.abstractBackground: Majority of bladder cancer deaths are caused due to transitional cell carcinoma (TCC) which is the most prevalent and chemoresistant malignancy of urinary bladder. Therefore, we analyzed the role of Sperm associated antigen 9 (SPAG9) in bladder TCC. Methodology and Findings: We examined SPAG9 expression and humoral response in 125 bladder TCC patients. Four bladder cancer cell lines were assessed for SPAG9 expression. In addition, we investigated the effect of SPAG9 ablation on cellular proliferation, cell cycle, migration and invasion in UM-UC-3 bladder cancer cells by employing gene silencing approach. Our SPAG9 gene and protein expression analysis revealed SPAG9 expression in 81% of bladder TCC tissue specimens. High SPAG9 expression (>60% SPAG9 positive cells) was found to be significantly associated with superficial non-muscle invasive stage (P = 0.042) and low grade tumors (P = 0.002) suggesting SPAG9 putative role in early spread and tumorigenesis. Humoral response against SPAG9 was observed in 95% of patients found positive for SPAG9 expression. All four bladder cancer cell lines revealed SPAG9 expression. In addition, SPAG9 gene silencing in UM-UC-3 cells resulted in induction of G 0 -G 1 arrest characterized by up-regulation of p16 and p21 and consequent down-regulation of cyclin E, cyclin D and cyclin B, CDK4 and CDK1. Further, SPAG9 gene silencing also resulted in reduction in cellular growth, and migration and invasion ability of cancer cells in vitro. Conclusions: Collectively, our data in clinical specimens indicated that SPAG9 is potential biomarker and therapeutic target for bladder TCC. © 2013 Kanojia et al.
dc.sourceUnpaywall
dc.subjectcancer testis antigen
dc.subjectcyclin B
dc.subjectcyclin D
dc.subjectcyclin dependent kinase 1
dc.subjectcyclin dependent kinase 4
dc.subjectcyclin E
dc.subjectprotein p16
dc.subjectprotein p21
dc.subjectsperm associated antigen 9
dc.subjectunclassified drug
dc.subjectadult
dc.subjectantigen expression
dc.subjectarticle
dc.subjectcancer cell
dc.subjectcancer grading
dc.subjectcell cycle arrest
dc.subjectcell cycle G0 phase
dc.subjectcell cycle G1 phase
dc.subjectcell invasion
dc.subjectcell migration
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectfemale
dc.subjectgene expression regulation
dc.subjectgene silencing
dc.subjectgenetic association
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjecthumoral immunity
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmiddle aged
dc.subjectnon muscle invasive bladder cancer
dc.subjecttransitional cell carcinoma
dc.subjectupregulation
dc.subjectAdaptor Proteins, Signal Transducing
dc.subjectAdult
dc.subjectCarcinoma, Transitional Cell
dc.subjectCell Cycle
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectTumor Markers, Biological
dc.subjectUrinary Bladder Neoplasms
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.description.doi10.1371/journal.pone.0081348
dc.description.sourcetitlePLoS ONE
dc.description.volume8
dc.description.issue12
dc.description.pagee81348
dc.published.statePublished
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