Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2019.02.122
Title: Specificity of the ergothioneine transporter natively expressed in HeLa cells
Authors: TUCKER, RAJ
CHEAH, IK 
HALLIWELL, B 
Keywords: Ergothioneine
OCTN1
SLC22A4
Transporter
Uptake
Issue Date: 21-May-2019
Publisher: Elsevier BV
Citation: TUCKER, RAJ, CHEAH, IK, HALLIWELL, B (2019-05-21). Specificity of the ergothioneine transporter natively expressed in HeLa cells. Biochemical and Biophysical Research Communications 513 (1) : 22-27. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2019.02.122
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: © 2019 Elsevier Inc. Ergothioneine is a biologically important compound that has been shown to be transported by the organic cation transporter novel type 1 (OCTN1). Following this discovery, a variety of alternate functions for OCTN1 have been suggested including an integral function in the extra-neuronal cholinergic system. The present study reaffirms the primacy of ergothioneine over these alternate substrates using natively expressed OCTN1 in HeLa cells. Besides the general transport inhibitors, quinidine, verapamil and pyrilamine no other putative substrate inhibited ergothioneine transport significantly, with only a slight inhibition demonstrated by carnitine. Even compounds structurally similar to ergothioneine failed to inhibit ergothioneine uptake, suggesting high selectivity of OCTN1. Ergothioneine was found to be avidly accumulated even at low concentrations (300 nM) by HeLa cells.
Source Title: Biochemical and Biophysical Research Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/155397
ISSN: 0006291X
10902104
DOI: 10.1016/j.bbrc.2019.02.122
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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