Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2019.02.122
DC FieldValue
dc.titleSpecificity of the ergothioneine transporter natively expressed in HeLa cells
dc.contributor.authorTUCKER, RAJ
dc.contributor.authorCHEAH, IK
dc.contributor.authorHALLIWELL, B
dc.date.accessioned2019-06-07T02:08:59Z
dc.date.available2019-06-07T02:08:59Z
dc.date.issued2019-05-21
dc.identifier.citationTUCKER, RAJ, CHEAH, IK, HALLIWELL, B (2019-05-21). Specificity of the ergothioneine transporter natively expressed in HeLa cells. Biochemical and Biophysical Research Communications 513 (1) : 22-27. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2019.02.122
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/155397
dc.description.abstract© 2019 Elsevier Inc. Ergothioneine is a biologically important compound that has been shown to be transported by the organic cation transporter novel type 1 (OCTN1). Following this discovery, a variety of alternate functions for OCTN1 have been suggested including an integral function in the extra-neuronal cholinergic system. The present study reaffirms the primacy of ergothioneine over these alternate substrates using natively expressed OCTN1 in HeLa cells. Besides the general transport inhibitors, quinidine, verapamil and pyrilamine no other putative substrate inhibited ergothioneine transport significantly, with only a slight inhibition demonstrated by carnitine. Even compounds structurally similar to ergothioneine failed to inhibit ergothioneine uptake, suggesting high selectivity of OCTN1. Ergothioneine was found to be avidly accumulated even at low concentrations (300 nM) by HeLa cells.
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceElements
dc.subjectErgothioneine
dc.subjectOCTN1
dc.subjectSLC22A4
dc.subjectTransporter
dc.subjectUptake
dc.typeArticle
dc.date.updated2019-06-04T03:51:47Z
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.doi10.1016/j.bbrc.2019.02.122
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume513
dc.description.issue1
dc.description.page22-27
dc.published.statePublished
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