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|Title:||Modeling type 3 long QT syndrome with cardiomyocytes derived from patient-specific induced pluripotent stem cells||Authors:||Ma D.
Induced pluripotent stem cells
Long QT syndrome
|Issue Date:||2013||Citation:||Ma D., Wei H., Zhao Y., Lu J., Li G., Sahib N.B.E., Tan T.H., Wong K.Y., Shim W., Wong P., Cook S.A., Liew R. (2013). Modeling type 3 long QT syndrome with cardiomyocytes derived from patient-specific induced pluripotent stem cells. International Journal of Cardiology 168 (6) : 5277-5286. ScholarBank@NUS Repository.||Abstract:||Background Type 3 long QT syndrome (LQT3) is the third most common form of LQT syndrome and is characterized by QT-interval prolongation resulting from a gain-of-function mutation in SCN5A. We aimed to establish a patient-specific human induced pluripotent stem cell (hiPSC) model of LQT3, which could be used for future drug testing and development of novel treatments for this inherited disorder. Methods and results Dermal fibroblasts obtained from a patient with LQT3 harboring a SCN5A mutation (c.5287G > A; p.V1763M) were reprogrammed to hiPSCs via repeated transfection of mRNA encoding OCT-4, SOX-2, KLF-4, C-MYC and LIN-28. hiPSC-derived cardiomyocytes (hiPSC-CMs) were obtained via cardiac differentiation. hiPSC-CMs derived from the patient's healthy sister were used as a control. Compared to the control, patient hiPSC-CMs exhibited dominant mutant SCN5A allele gene expression, significantly prolonged action potential duration or APD (paced CMs of control vs. patient: 226.50 � 17.89 ms vs. 536.59 � 37.1 ms; mean � SEM, p < 0.005), an increased tetrodotoxin (TTX)-sensitive late or persistent Na+ current (control vs. patient: 0.65 � 0.11 vs. 3.16 � 0.27 pA/pF; n = 9, p < 0.01), a positive shift of steady state inactivation and a faster recovery from inactivation. Mexiletine, a NaV1.5 blocker, reversed the elevated late Na + current and prolonged APD in LQT3 hiPSC-CMs. Conclusions We demonstrate that hiPSC-CMs derived from a LQT3 patient recapitulate the biophysical abnormalities that define LQT3. The clinical significance of such an in vitro model is in the development of novel therapeutic strategies and a more personalized approach in testing drugs on patients with LQT3. ? 2013 Elsevier Ireland Ltd ? 2013 Published by Elsevier Ireland Ltd.||Source Title:||International Journal of Cardiology||URI:||http://scholarbank.nus.edu.sg/handle/10635/150867||ISSN:||1675273|
|Appears in Collections:||Staff Publications|
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