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Title: Human finger-prick induced pluripotent stem cells facilitate the development of stem cell banking
Authors: Tan H.-K.
Toh C.-X.D.
Ma D. 
Yang B.
Liu T.M. 
Lu J.
Wong C.-W. 
Tan T.-K.
Li H.
Syn C. 
Tan E.-L. 
Lim B. 
Lim Y.-P. 
Cook S.A. 
Loh Y.-H. 
Keywords: Finger prick
Human induced pluripotent stem cell banking
Human peripheral blood
Induced pluripotent stem cells
Issue Date: 2014
Publisher: John Wiley and Sons Ltd
Citation: Tan H.-K., Toh C.-X.D., Ma D., Yang B., Liu T.M., Lu J., Wong C.-W., Tan T.-K., Li H., Syn C., Tan E.-L., Lim B., Lim Y.-P., Cook S.A., Loh Y.-H. (2014). Human finger-prick induced pluripotent stem cells facilitate the development of stem cell banking. Stem Cells Translational Medicine 3 (5) : 586-598. ScholarBank@NUS Repository.
Abstract: Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients can be a good model for studying human diseases and for future therapeutic regenerative medicine. Current initiatives to establish human iPSC (hiPSC) banking face challenges in recruiting large numbers of donors with diverse diseased, genetic, and phenotypic representations. In this study, we describe the efficient derivation of transgene-free hiPSCs from human finger-prick blood. Finger-prick sample collection can be performed on a "do-it-yourself" basis by donors and sent to the hiPSC facility for reprogramming. We show that single-drop volumes of finger-prick samples are sufficient for performing cellular reprogramming, DNA sequencing, and blood serotyping in parallel. Our novel strategy has the potential to facilitate the development of large-scale hiPSC banking worldwide. ? AlphaMed Press 2014.
Source Title: Stem Cells Translational Medicine
ISSN: 21576564
DOI: 10.5966/sctm.2013-0195
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