Development of a human induced pluripotent stem cell (iPSC) line from a Parkinson's disease patient carrying the N551K variant in LRRK2 gene

Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.scr.2016.12.013

Title:	Development of a human induced pluripotent stem cell (iPSC) line from a Parkinson's disease patient carrying the N551K variant in LRRK2 gene
Authors:	Ma D. Ng E.Y. Zeng L. Lim C.Y.Y. Zhao Y. Tan E.K.
Issue Date:	2017
Publisher:	Elsevier B.V.
Citation:	Ma D., Ng E.Y., Zeng L., Lim C.Y.Y., Zhao Y., Tan E.K. (2017). Development of a human induced pluripotent stem cell (iPSC) line from a Parkinson's disease patient carrying the N551K variant in LRRK2 gene. Stem Cell Research 18 : 51-53. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2016.12.013
Abstract:	Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with N551K variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model can complement in vivo PD models for pathophysiological studies and drug screening. © 2016 The Authors
Source Title:	Stem Cell Research
URI:	http://scholarbank.nus.edu.sg/handle/10635/148967
ISSN:	18735061
DOI:	10.1016/j.scr.2016.12.013
Appears in Collections:	Staff Publications Elements

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