Please use this identifier to cite or link to this item:
Title: The use of amniotic fluid and serum with propanediol in freezing of murine 2-cell embryos
Authors: Ng, S.C. 
Sathananthan, H.
Bongso, A. 
Lee, M.-N.
Mok, H.
Wong, P.-C.
Ratnam, S.S. 
Issue Date: 1988
Citation: Ng, S.C., Sathananthan, H., Bongso, A., Lee, M.-N., Mok, H., Wong, P.-C., Ratnam, S.S. (1988). The use of amniotic fluid and serum with propanediol in freezing of murine 2-cell embryos. Fertility and Sterility 50 (3) : 510-513. ScholarBank@NUS Repository.
Abstract: Human and mouse embryos have been cultured in amniotic fluid (AF). Human AF and human serum (HS) are used in the freeze-thaw of 2-cell mouse embryos. Two hundred seventy-five 2-cell embryos were collected into phosphate-buffered saline with 20% HS and 20% AF and into 100% HS and AF. The embryos were cooled with propanediol as cyroprotectant at a controlled rate. After thaw, they were cultured in T6 with 3 mg/ml bovine serum albumin. Blastocyst formation post-thaw was 56/79, 44/70, 51/61, and 56/79 of intact embryos from 20% HS, 20% AF, pure HS, and pure AF (NS). But blastocyst hatching was better from embryos frozen in pure HS (22/61, compared with 16/79 for 20% HS; P < 0.05). Hence there is not advantage in using AF in freeze-thaw, but pure HS may be of use.
Source Title: Fertility and Sterility
ISSN: 00150282
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Nov 24, 2022

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.