Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/133692
Title: The use of amniotic fluid and serum with propanediol in freezing of murine 2-cell embryos
Authors: Ng, S.C. 
Sathananthan, H.
Bongso, A. 
Lee, M.-N.
Mok, H.
Wong, P.-C.
Ratnam, S.S. 
Issue Date: 1988
Source: Ng, S.C., Sathananthan, H., Bongso, A., Lee, M.-N., Mok, H., Wong, P.-C., Ratnam, S.S. (1988). The use of amniotic fluid and serum with propanediol in freezing of murine 2-cell embryos. Fertility and Sterility 50 (3) : 510-513. ScholarBank@NUS Repository.
Abstract: Human and mouse embryos have been cultured in amniotic fluid (AF). Human AF and human serum (HS) are used in the freeze-thaw of 2-cell mouse embryos. Two hundred seventy-five 2-cell embryos were collected into phosphate-buffered saline with 20% HS and 20% AF and into 100% HS and AF. The embryos were cooled with propanediol as cyroprotectant at a controlled rate. After thaw, they were cultured in T6 with 3 mg/ml bovine serum albumin. Blastocyst formation post-thaw was 56/79, 44/70, 51/61, and 56/79 of intact embryos from 20% HS, 20% AF, pure HS, and pure AF (NS). But blastocyst hatching was better from embryos frozen in pure HS (22/61, compared with 16/79 for 20% HS; P < 0.05). Hence there is not advantage in using AF in freeze-thaw, but pure HS may be of use.
Source Title: Fertility and Sterility
URI: http://scholarbank.nus.edu.sg/handle/10635/133692
ISSN: 00150282
Appears in Collections:Staff Publications

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