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Title: Development of a high sensitivity rapid sandwich ELISA procedure and its comparison with the conventional approach
Authors: Dixit, C.K.
Vashist, S.K. 
O'neill, F.T.
O'reilly, B.
MacCraith, B.D.
O'kennedy, R.
Issue Date: 15-Aug-2010
Citation: Dixit, C.K., Vashist, S.K., O'neill, F.T., O'reilly, B., MacCraith, B.D., O'kennedy, R. (2010-08-15). Development of a high sensitivity rapid sandwich ELISA procedure and its comparison with the conventional approach. Analytical Chemistry 82 (16) : 7049-7052. ScholarBank@NUS Repository.
Abstract: A highly sensitive and rapid sandwich enzyme-linked immunosorbent assay (ELISA) procedure was developed for the detection of human fetuin A/AHSG (α2-HS-glycoprotein), a specific biomarker for hepatocellular carcinoma and atherosclerosis. Anti-human fetuin A antibody was immobilized on aminopropyltriethoxysilane-mediated amine-functionalized microtiter plates using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and N-hydroxysulfosuccinimide-based heterobifunctional cross-linking. The analytical sensitivity of the developed assay was 39 pg/mL, compared to 625 pg/mL for the conventional assay. The generic nature of the developed procedure was demonstrated by performing human fetuin A assays on different polymeric matrixes, i.e., polystyrene, poly(methyl methacrylate), and polycyclo-olefin (Zeonex), in a modified microtiter plate format. Thus, the newly developed procedure has considerable advantages over the existing method. © 2010 American Chemical Society.
Source Title: Analytical Chemistry
ISSN: 00032700
DOI: 10.1021/ac101339q
Appears in Collections:Staff Publications

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