Please use this identifier to cite or link to this item:
|Title:||Development of a high sensitivity rapid sandwich ELISA procedure and its comparison with the conventional approach|
|Citation:||Dixit, C.K., Vashist, S.K., O'neill, F.T., O'reilly, B., MacCraith, B.D., O'kennedy, R. (2010-08-15). Development of a high sensitivity rapid sandwich ELISA procedure and its comparison with the conventional approach. Analytical Chemistry 82 (16) : 7049-7052. ScholarBank@NUS Repository. https://doi.org/10.1021/ac101339q|
|Abstract:||A highly sensitive and rapid sandwich enzyme-linked immunosorbent assay (ELISA) procedure was developed for the detection of human fetuin A/AHSG (α2-HS-glycoprotein), a specific biomarker for hepatocellular carcinoma and atherosclerosis. Anti-human fetuin A antibody was immobilized on aminopropyltriethoxysilane-mediated amine-functionalized microtiter plates using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and N-hydroxysulfosuccinimide-based heterobifunctional cross-linking. The analytical sensitivity of the developed assay was 39 pg/mL, compared to 625 pg/mL for the conventional assay. The generic nature of the developed procedure was demonstrated by performing human fetuin A assays on different polymeric matrixes, i.e., polystyrene, poly(methyl methacrylate), and polycyclo-olefin (Zeonex), in a modified microtiter plate format. Thus, the newly developed procedure has considerable advantages over the existing method. © 2010 American Chemical Society.|
|Source Title:||Analytical Chemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 21, 2018
WEB OF SCIENCETM
checked on Apr 30, 2018
checked on May 3, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.