Please use this identifier to cite or link to this item: https://doi.org/10.1021/ac101339q
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dc.titleDevelopment of a high sensitivity rapid sandwich ELISA procedure and its comparison with the conventional approach
dc.contributor.authorDixit, C.K.
dc.contributor.authorVashist, S.K.
dc.contributor.authorO'neill, F.T.
dc.contributor.authorO'reilly, B.
dc.contributor.authorMacCraith, B.D.
dc.contributor.authorO'kennedy, R.
dc.date.accessioned2016-10-18T06:27:37Z
dc.date.available2016-10-18T06:27:37Z
dc.date.issued2010-08-15
dc.identifier.citationDixit, C.K., Vashist, S.K., O'neill, F.T., O'reilly, B., MacCraith, B.D., O'kennedy, R. (2010-08-15). Development of a high sensitivity rapid sandwich ELISA procedure and its comparison with the conventional approach. Analytical Chemistry 82 (16) : 7049-7052. ScholarBank@NUS Repository. https://doi.org/10.1021/ac101339q
dc.identifier.issn00032700
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128549
dc.description.abstractA highly sensitive and rapid sandwich enzyme-linked immunosorbent assay (ELISA) procedure was developed for the detection of human fetuin A/AHSG (α2-HS-glycoprotein), a specific biomarker for hepatocellular carcinoma and atherosclerosis. Anti-human fetuin A antibody was immobilized on aminopropyltriethoxysilane-mediated amine-functionalized microtiter plates using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and N-hydroxysulfosuccinimide-based heterobifunctional cross-linking. The analytical sensitivity of the developed assay was 39 pg/mL, compared to 625 pg/mL for the conventional assay. The generic nature of the developed procedure was demonstrated by performing human fetuin A assays on different polymeric matrixes, i.e., polystyrene, poly(methyl methacrylate), and polycyclo-olefin (Zeonex), in a modified microtiter plate format. Thus, the newly developed procedure has considerable advantages over the existing method. © 2010 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/ac101339q
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.description.doi10.1021/ac101339q
dc.description.sourcetitleAnalytical Chemistry
dc.description.volume82
dc.description.issue16
dc.description.page7049-7052
dc.description.codenANCHA
dc.identifier.isiut000280758400041
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