Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers

Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jbiosc.2013.11.011

Title:	Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers
Authors:	Higuchi, S. Watanabe, T.M. Kawauchi, K. Ichimura, T. Fujita, H.
Keywords:	Actin filament Elasticity Fibroblasts Induced pluripotent stem cells Reprogramming
Issue Date:	2014
Citation:	Higuchi, S., Watanabe, T.M., Kawauchi, K., Ichimura, T., Fujita, H. (2014). Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers. Journal of Bioscience and Bioengineering 117 (6) : 749-755. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jbiosc.2013.11.011
Abstract:	Substrate elasticity is a potent regulator of the cell state. Soft substrates have been shown to promote the homogeneous self-renewal of mouse embryonic stem cells through the down-regulation of cell-matrix tractions. We therefore investigated whether soft substrates promote the reprogramming of somatic cells into induced pluripotent stem (iPS) cells. After retroviral infection with five factors, Oct3/4, Klf4, Sox2, Lin28 and Nanog, mouse embryonic fibroblasts (MEFs) were cultured on several artificial substrates of varying elasticity and examined for the expression of pluripotency genes. When MEFs were cultured on soft (
Source Title:	Journal of Bioscience and Bioengineering
URI:	http://scholarbank.nus.edu.sg/handle/10635/126611
ISSN:	13474421
DOI:	10.1016/j.jbiosc.2013.11.011
Appears in Collections:	Staff Publications

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