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Title: Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers
Authors: Higuchi, S.
Watanabe, T.M.
Kawauchi, K. 
Ichimura, T.
Fujita, H.
Keywords: Actin filament
Induced pluripotent stem cells
Issue Date: 2014
Citation: Higuchi, S., Watanabe, T.M., Kawauchi, K., Ichimura, T., Fujita, H. (2014). Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers. Journal of Bioscience and Bioengineering 117 (6) : 749-755. ScholarBank@NUS Repository.
Abstract: Substrate elasticity is a potent regulator of the cell state. Soft substrates have been shown to promote the homogeneous self-renewal of mouse embryonic stem cells through the down-regulation of cell-matrix tractions. We therefore investigated whether soft substrates promote the reprogramming of somatic cells into induced pluripotent stem (iPS) cells. After retroviral infection with five factors, Oct3/4, Klf4, Sox2, Lin28 and Nanog, mouse embryonic fibroblasts (MEFs) were cultured on several artificial substrates of varying elasticity and examined for the expression of pluripotency genes. When MEFs were cultured on soft (
Source Title: Journal of Bioscience and Bioengineering
ISSN: 13474421
DOI: 10.1016/j.jbiosc.2013.11.011
Appears in Collections:Staff Publications

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