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Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jbiosc.2013.11.011
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dc.titleCulturing of mouse and human cells on soft substrates promote the expression of stem cell markers
dc.contributor.authorHiguchi, S.
dc.contributor.authorWatanabe, T.M.
dc.contributor.authorKawauchi, K.
dc.contributor.authorIchimura, T.
dc.contributor.authorFujita, H.
dc.date.accessioned2016-09-06T05:43:53Z
dc.date.available2016-09-06T05:43:53Z
dc.date.issued2014
dc.identifier.citationHiguchi, S., Watanabe, T.M., Kawauchi, K., Ichimura, T., Fujita, H. (2014). Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers. Journal of Bioscience and Bioengineering 117 (6) : 749-755. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jbiosc.2013.11.011
dc.identifier.issn13474421
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/126611
dc.description.abstractSubstrate elasticity is a potent regulator of the cell state. Soft substrates have been shown to promote the homogeneous self-renewal of mouse embryonic stem cells through the down-regulation of cell-matrix tractions. We therefore investigated whether soft substrates promote the reprogramming of somatic cells into induced pluripotent stem (iPS) cells. After retroviral infection with five factors, Oct3/4, Klf4, Sox2, Lin28 and Nanog, mouse embryonic fibroblasts (MEFs) were cultured on several artificial substrates of varying elasticity and examined for the expression of pluripotency genes. When MEFs were cultured on soft (
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jbiosc.2013.11.011
dc.sourceScopus
dc.subjectActin filament
dc.subjectElasticity
dc.subjectFibroblasts
dc.subjectInduced pluripotent stem cells
dc.subjectReprogramming
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.jbiosc.2013.11.011
dc.description.sourcetitleJournal of Bioscience and Bioengineering
dc.description.volume117
dc.description.issue6
dc.description.page749-755
dc.description.codenJBBIF
dc.identifier.isiut000339219400017
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