Please use this identifier to cite or link to this item:
|Title:||Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers|
Induced pluripotent stem cells
|Citation:||Higuchi, S., Watanabe, T.M., Kawauchi, K., Ichimura, T., Fujita, H. (2014). Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers. Journal of Bioscience and Bioengineering 117 (6) : 749-755. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jbiosc.2013.11.011|
|Abstract:||Substrate elasticity is a potent regulator of the cell state. Soft substrates have been shown to promote the homogeneous self-renewal of mouse embryonic stem cells through the down-regulation of cell-matrix tractions. We therefore investigated whether soft substrates promote the reprogramming of somatic cells into induced pluripotent stem (iPS) cells. After retroviral infection with five factors, Oct3/4, Klf4, Sox2, Lin28 and Nanog, mouse embryonic fibroblasts (MEFs) were cultured on several artificial substrates of varying elasticity and examined for the expression of pluripotency genes. When MEFs were cultured on soft (|
|Source Title:||Journal of Bioscience and Bioengineering|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 14, 2018
WEB OF SCIENCETM
checked on Nov 21, 2018
checked on Dec 7, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.