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|Title:||Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers|
Induced pluripotent stem cells
|Source:||Higuchi, S., Watanabe, T.M., Kawauchi, K., Ichimura, T., Fujita, H. (2014). Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers. Journal of Bioscience and Bioengineering 117 (6) : 749-755. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jbiosc.2013.11.011|
|Abstract:||Substrate elasticity is a potent regulator of the cell state. Soft substrates have been shown to promote the homogeneous self-renewal of mouse embryonic stem cells through the down-regulation of cell-matrix tractions. We therefore investigated whether soft substrates promote the reprogramming of somatic cells into induced pluripotent stem (iPS) cells. After retroviral infection with five factors, Oct3/4, Klf4, Sox2, Lin28 and Nanog, mouse embryonic fibroblasts (MEFs) were cultured on several artificial substrates of varying elasticity and examined for the expression of pluripotency genes. When MEFs were cultured on soft (|
|Source Title:||Journal of Bioscience and Bioengineering|
|Appears in Collections:||Staff Publications|
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