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|Title:||Structural changes in dengue virus when exposed to a temperature of 37°C||Authors:||Fibriansah, G.
|Issue Date:||Jul-2013||Citation:||Fibriansah, G., Ng, T.-S., Kostyuchenko, V.A., Lee, J., Lee, S., Wang, J., Lok, S.-M. (2013-07). Structural changes in dengue virus when exposed to a temperature of 37°C. Journal of Virology 87 (13) : 7585-7592. ScholarBank@NUS Repository. https://doi.org/10.1128/JVI.00757-13||Abstract:||Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest that insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This was confirmed by our cryo-electron microscopy images of dengue virus incubated at 37°C, where viruses change their surface from smooth to rough. Here we present the cryo-electron microscopy structures of dengue virus at 37°C. Image analysis showed four classes of particles. The three-dimensional (3D) map of one of these classes, representing half of the imaged virus population, shows that the E protein shell has expanded and there is a hole at the 3-fold vertices. Fitting E protein structures into the map suggests that all of the interdimeric and some intradimeric E protein interactions are weakened. The accessibility of some previously found cryptic epitopes on this class of particles is discussed. © 2013, American Society for Microbiology.||Source Title:||Journal of Virology||URI:||http://scholarbank.nus.edu.sg/handle/10635/124734||ISSN:||0022538X||DOI:||10.1128/JVI.00757-13|
|Appears in Collections:||Staff Publications|
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