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Title: Structural changes in dengue virus when exposed to a temperature of 37°C
Authors: Fibriansah, G. 
Ng, T.-S. 
Kostyuchenko, V.A. 
Lee, J. 
Lee, S.
Wang, J.
Lok, S.-M. 
Issue Date: Jul-2013
Citation: Fibriansah, G., Ng, T.-S., Kostyuchenko, V.A., Lee, J., Lee, S., Wang, J., Lok, S.-M. (2013-07). Structural changes in dengue virus when exposed to a temperature of 37°C. Journal of Virology 87 (13) : 7585-7592. ScholarBank@NUS Repository.
Abstract: Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest that insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This was confirmed by our cryo-electron microscopy images of dengue virus incubated at 37°C, where viruses change their surface from smooth to rough. Here we present the cryo-electron microscopy structures of dengue virus at 37°C. Image analysis showed four classes of particles. The three-dimensional (3D) map of one of these classes, representing half of the imaged virus population, shows that the E protein shell has expanded and there is a hole at the 3-fold vertices. Fitting E protein structures into the map suggests that all of the interdimeric and some intradimeric E protein interactions are weakened. The accessibility of some previously found cryptic epitopes on this class of particles is discussed. © 2013, American Society for Microbiology.
Source Title: Journal of Virology
ISSN: 0022538X
DOI: 10.1128/JVI.00757-13
Appears in Collections:Staff Publications

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