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Title: Ablation of phosphoinositide-3-kinase class II alpha suppresses hepatoma cell proliferation
Authors: Ng, S.K.L.
Neo, S.-Y.
Yap, Y.-W.
Karuturi, R.K.M.
Loh, E.S.L.
Liau, K.-H.
Ren, E.-C. 
Keywords: Apoptosis
Cell growth
Issue Date: 18-Sep-2009
Citation: Ng, S.K.L., Neo, S.-Y., Yap, Y.-W., Karuturi, R.K.M., Loh, E.S.L., Liau, K.-H., Ren, E.-C. (2009-09-18). Ablation of phosphoinositide-3-kinase class II alpha suppresses hepatoma cell proliferation. Biochemical and Biophysical Research Communications 387 (2) : 310-315. ScholarBank@NUS Repository.
Abstract: Cancer such as hepatocellular carcinoma (HCC) is characterized by complex perturbations in multiple signaling pathways, including the phosphoinositide-3-kinase (PI3K/AKT) pathways. Herein we investigated the role of PI3K catalytic isoforms, particularly class II isoforms in HCC proliferation. Among the siRNAs tested against the eight known catalytic PI3K isoforms, specific ablation of class II PI3K alpha (PIK3C2α) was the most effective in impairing cell growth and this was accompanied by concomitant decrease in PIK3C2α mRNA and protein levels. Colony formation ability of cells deficient for PIK3C2α was markedly reduced and growth arrest was associated with increased caspase 3 levels. A small but significant difference in gene dosage and expression levels was detected between tumor and non-tumor tissues in a cohort of 19 HCC patients. Taken together, these data suggest for the first time that in addition to class I PI3Ks in cancer, class II PIK3C2α can modulate HCC cell growth. © 2009 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2009.07.013
Appears in Collections:Staff Publications

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