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https://scholarbank.nus.edu.sg/handle/10635/112041
| Title: | Recombinant human tumor necrosis factor-β entrapped in liposomes formed by a modification of the dehydration-rehydration method retains potent cytotoxic activity on L929 cells in vitro | Authors: | Tan, L. Goh, C.R. Porter, A.G. |
Keywords: | L929 cells Liposomes Tumor necrosis factor-β |
Issue Date: | 1992 | Citation: | Tan, L.,Goh, C.R.,Porter, A.G. (1992). Recombinant human tumor necrosis factor-β entrapped in liposomes formed by a modification of the dehydration-rehydration method retains potent cytotoxic activity on L929 cells in vitro. Japanese Journal of Pharmacology 59 (2) : 235-238. ScholarBank@NUS Repository. | Abstract: | Recombinant human tumor necrosis factor-β (rhTNF-β) may be encapsulated with high efficiency in phosphatidylcholine and distearoylphosphatidylcholine liposomes, with entrapment values of 93.4% and 92.3%, respectively, by first entrapping the substance in multilamellar vesicles using a high solute-to-phospholipid ratio followed by freeze-drying and then rehydration. The entrapped cytokine retains potent cytotoxic activity on L929 cells in vitro, causing 100% cytotoxicity, equal to that of free rhTNF-β at a concentration of about 5 X 10-8g/ml. | Source Title: | Japanese Journal of Pharmacology | URI: | http://scholarbank.nus.edu.sg/handle/10635/112041 | ISSN: | 00215198 |
| Appears in Collections: | Staff Publications |
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