Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/112041
Title: Recombinant human tumor necrosis factor-β entrapped in liposomes formed by a modification of the dehydration-rehydration method retains potent cytotoxic activity on L929 cells in vitro
Authors: Tan, L.
Goh, C.R. 
Porter, A.G. 
Keywords: L929 cells
Liposomes
Tumor necrosis factor-β
Issue Date: 1992
Source: Tan, L.,Goh, C.R.,Porter, A.G. (1992). Recombinant human tumor necrosis factor-β entrapped in liposomes formed by a modification of the dehydration-rehydration method retains potent cytotoxic activity on L929 cells in vitro. Japanese Journal of Pharmacology 59 (2) : 235-238. ScholarBank@NUS Repository.
Abstract: Recombinant human tumor necrosis factor-β (rhTNF-β) may be encapsulated with high efficiency in phosphatidylcholine and distearoylphosphatidylcholine liposomes, with entrapment values of 93.4% and 92.3%, respectively, by first entrapping the substance in multilamellar vesicles using a high solute-to-phospholipid ratio followed by freeze-drying and then rehydration. The entrapped cytokine retains potent cytotoxic activity on L929 cells in vitro, causing 100% cytotoxicity, equal to that of free rhTNF-β at a concentration of about 5 X 10-8g/ml.
Source Title: Japanese Journal of Pharmacology
URI: http://scholarbank.nus.edu.sg/handle/10635/112041
ISSN: 00215198
Appears in Collections:Staff Publications

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