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|Title:||Effects of NH4Cl and nocodazole on polarized fibronectin secretion vary amongst different epithelial cell types.||Authors:||Low, S.H.
|Issue Date:||Jan-1994||Citation:||Low, S.H.,Wong, S.H.,Tang, B.L.,Hong, W. (1994-01). Effects of NH4Cl and nocodazole on polarized fibronectin secretion vary amongst different epithelial cell types.. Molecular membrane biology 11 (1) : 45-54. ScholarBank@NUS Repository.||Abstract:||The extracellular matrix protein fibronectin was found to be secreted by three polarized epithelial cell lines Madin-Darby canine kidney (MDCK), Caco-2 and LLC-PK1. About 54 and 46% of fibronectin was secreted from the apical and basolateral cell surfaces, respectively, in MDCK cells. In Caco-2 and LLC-PK1 cells, the majority (about 92-93%) of fibronectin secretion occurs from the basolateral cell surface, with the remaining 7-8% from the apical surface. In all three cell types, NH4Cl was found to inhibit basolateral secretion (resulting in enhanced apical secretion), while total fibronectin secretion was not significantly affected (although a delay in secretion was observed). Nocodazole reduced total fibronectin secretion to about 70% of control levels in MDCK and Caco-2 cells, with significant inhibition on secretion from both surfaces. In contrast, total fibronectin secretion was enhanced by nocodazole in LLC-PK1 cells. Furthermore, the majority of fibronectin secretion was redirected to the apical cell surface in LLC-PK1 cells. These observations demonstrate that the nature as well as the extent of the effects of NH4-Cl and nocodazole on polarized fibronectin secretion varies amongst different epithelial cell types.||Source Title:||Molecular membrane biology||URI:||http://scholarbank.nus.edu.sg/handle/10635/111865||ISSN:||09687688|
|Appears in Collections:||Staff Publications|
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