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Title: Effects of NH4Cl and nocodazole on polarized fibronectin secretion vary amongst different epithelial cell types.
Authors: Low, S.H. 
Wong, S.H. 
Tang, B.L. 
Hong, W. 
Issue Date: Jan-1994
Citation: Low, S.H.,Wong, S.H.,Tang, B.L.,Hong, W. (1994-01). Effects of NH4Cl and nocodazole on polarized fibronectin secretion vary amongst different epithelial cell types.. Molecular membrane biology 11 (1) : 45-54. ScholarBank@NUS Repository.
Abstract: The extracellular matrix protein fibronectin was found to be secreted by three polarized epithelial cell lines Madin-Darby canine kidney (MDCK), Caco-2 and LLC-PK1. About 54 and 46% of fibronectin was secreted from the apical and basolateral cell surfaces, respectively, in MDCK cells. In Caco-2 and LLC-PK1 cells, the majority (about 92-93%) of fibronectin secretion occurs from the basolateral cell surface, with the remaining 7-8% from the apical surface. In all three cell types, NH4Cl was found to inhibit basolateral secretion (resulting in enhanced apical secretion), while total fibronectin secretion was not significantly affected (although a delay in secretion was observed). Nocodazole reduced total fibronectin secretion to about 70% of control levels in MDCK and Caco-2 cells, with significant inhibition on secretion from both surfaces. In contrast, total fibronectin secretion was enhanced by nocodazole in LLC-PK1 cells. Furthermore, the majority of fibronectin secretion was redirected to the apical cell surface in LLC-PK1 cells. These observations demonstrate that the nature as well as the extent of the effects of NH4-Cl and nocodazole on polarized fibronectin secretion varies amongst different epithelial cell types.
Source Title: Molecular membrane biology
ISSN: 09687688
Appears in Collections:Staff Publications

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