Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/111865
DC Field | Value | |
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dc.title | Effects of NH4Cl and nocodazole on polarized fibronectin secretion vary amongst different epithelial cell types. | |
dc.contributor.author | Low, S.H. | |
dc.contributor.author | Wong, S.H. | |
dc.contributor.author | Tang, B.L. | |
dc.contributor.author | Hong, W. | |
dc.date.accessioned | 2014-11-28T02:50:32Z | |
dc.date.available | 2014-11-28T02:50:32Z | |
dc.date.issued | 1994-01 | |
dc.identifier.citation | Low, S.H.,Wong, S.H.,Tang, B.L.,Hong, W. (1994-01). Effects of NH4Cl and nocodazole on polarized fibronectin secretion vary amongst different epithelial cell types.. Molecular membrane biology 11 (1) : 45-54. ScholarBank@NUS Repository. | |
dc.identifier.issn | 09687688 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/111865 | |
dc.description.abstract | The extracellular matrix protein fibronectin was found to be secreted by three polarized epithelial cell lines Madin-Darby canine kidney (MDCK), Caco-2 and LLC-PK1. About 54 and 46% of fibronectin was secreted from the apical and basolateral cell surfaces, respectively, in MDCK cells. In Caco-2 and LLC-PK1 cells, the majority (about 92-93%) of fibronectin secretion occurs from the basolateral cell surface, with the remaining 7-8% from the apical surface. In all three cell types, NH4Cl was found to inhibit basolateral secretion (resulting in enhanced apical secretion), while total fibronectin secretion was not significantly affected (although a delay in secretion was observed). Nocodazole reduced total fibronectin secretion to about 70% of control levels in MDCK and Caco-2 cells, with significant inhibition on secretion from both surfaces. In contrast, total fibronectin secretion was enhanced by nocodazole in LLC-PK1 cells. Furthermore, the majority of fibronectin secretion was redirected to the apical cell surface in LLC-PK1 cells. These observations demonstrate that the nature as well as the extent of the effects of NH4-Cl and nocodazole on polarized fibronectin secretion varies amongst different epithelial cell types. | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.sourcetitle | Molecular membrane biology | |
dc.description.volume | 11 | |
dc.description.issue | 1 | |
dc.description.page | 45-54 | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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