Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/111865
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dc.titleEffects of NH4Cl and nocodazole on polarized fibronectin secretion vary amongst different epithelial cell types.
dc.contributor.authorLow, S.H.
dc.contributor.authorWong, S.H.
dc.contributor.authorTang, B.L.
dc.contributor.authorHong, W.
dc.date.accessioned2014-11-28T02:50:32Z
dc.date.available2014-11-28T02:50:32Z
dc.date.issued1994-01
dc.identifier.citationLow, S.H.,Wong, S.H.,Tang, B.L.,Hong, W. (1994-01). Effects of NH4Cl and nocodazole on polarized fibronectin secretion vary amongst different epithelial cell types.. Molecular membrane biology 11 (1) : 45-54. ScholarBank@NUS Repository.
dc.identifier.issn09687688
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/111865
dc.description.abstractThe extracellular matrix protein fibronectin was found to be secreted by three polarized epithelial cell lines Madin-Darby canine kidney (MDCK), Caco-2 and LLC-PK1. About 54 and 46% of fibronectin was secreted from the apical and basolateral cell surfaces, respectively, in MDCK cells. In Caco-2 and LLC-PK1 cells, the majority (about 92-93%) of fibronectin secretion occurs from the basolateral cell surface, with the remaining 7-8% from the apical surface. In all three cell types, NH4Cl was found to inhibit basolateral secretion (resulting in enhanced apical secretion), while total fibronectin secretion was not significantly affected (although a delay in secretion was observed). Nocodazole reduced total fibronectin secretion to about 70% of control levels in MDCK and Caco-2 cells, with significant inhibition on secretion from both surfaces. In contrast, total fibronectin secretion was enhanced by nocodazole in LLC-PK1 cells. Furthermore, the majority of fibronectin secretion was redirected to the apical cell surface in LLC-PK1 cells. These observations demonstrate that the nature as well as the extent of the effects of NH4-Cl and nocodazole on polarized fibronectin secretion varies amongst different epithelial cell types.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.sourcetitleMolecular membrane biology
dc.description.volume11
dc.description.issue1
dc.description.page45-54
dc.identifier.isiutNOT_IN_WOS
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