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https://doi.org/10.1515/CCLM.2008.217
| Title: | An improved quality control for bisulfite-PCR-based DNA methylation analysis: Cycle threshold value | Authors: | Ang, P.W. Toh, H.B. Iacopetta, B. Soong, R. |
Keywords: | Bisulfite treatment DNA methylation Epigenetics Molecular diagnostics Quality control Real-time PCR |
Issue Date: | 1-Aug-2008 | Citation: | Ang, P.W., Toh, H.B., Iacopetta, B., Soong, R. (2008-08-01). An improved quality control for bisulfite-PCR-based DNA methylation analysis: Cycle threshold value. Clinical Chemistry and Laboratory Medicine 46 (8) : 1117-1121. ScholarBank@NUS Repository. https://doi.org/10.1515/CCLM.2008.217 | Abstract: | Background: The diagnostic potential of DNA methylation has generated a need for quality control systems in its analysis. Here, we tested whether cycle threshold (Ct) values from real-time quantitative PCR correlate better with the reliability of bisulfite-PCR methylation analyses than spectrophotometric concentrations. Methods: A total of 18 cell line DNA samples were quantified for β-actin (ACTB) Ct and spectrophotometric values from 1-μL volumes. A further 1 μL was analyzed in quintuplicate for bisulfite DNA ACTB by MethyLight. Correlations between Ct and spectrophotometric values, and MethyLight qualitative (detection in all replicates) and quantitative (Ct value standard deviation | Source Title: | Clinical Chemistry and Laboratory Medicine | URI: | http://scholarbank.nus.edu.sg/handle/10635/109173 | ISSN: | 14346621 | DOI: | 10.1515/CCLM.2008.217 |
| Appears in Collections: | Staff Publications |
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