Please use this identifier to cite or link to this item: https://doi.org/10.1515/CCLM.2008.217
Title: An improved quality control for bisulfite-PCR-based DNA methylation analysis: Cycle threshold value
Authors: Ang, P.W.
Toh, H.B.
Iacopetta, B.
Soong, R. 
Keywords: Bisulfite treatment
DNA methylation
Epigenetics
Molecular diagnostics
Quality control
Real-time PCR
Issue Date: 1-Aug-2008
Citation: Ang, P.W., Toh, H.B., Iacopetta, B., Soong, R. (2008-08-01). An improved quality control for bisulfite-PCR-based DNA methylation analysis: Cycle threshold value. Clinical Chemistry and Laboratory Medicine 46 (8) : 1117-1121. ScholarBank@NUS Repository. https://doi.org/10.1515/CCLM.2008.217
Abstract: Background: The diagnostic potential of DNA methylation has generated a need for quality control systems in its analysis. Here, we tested whether cycle threshold (Ct) values from real-time quantitative PCR correlate better with the reliability of bisulfite-PCR methylation analyses than spectrophotometric concentrations. Methods: A total of 18 cell line DNA samples were quantified for β-actin (ACTB) Ct and spectrophotometric values from 1-μL volumes. A further 1 μL was analyzed in quintuplicate for bisulfite DNA ACTB by MethyLight. Correlations between Ct and spectrophotometric values, and MethyLight qualitative (detection in all replicates) and quantitative (Ct value standard deviation
Source Title: Clinical Chemistry and Laboratory Medicine
URI: http://scholarbank.nus.edu.sg/handle/10635/109173
ISSN: 14346621
DOI: 10.1515/CCLM.2008.217
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

4
checked on Dec 12, 2018

WEB OF SCIENCETM
Citations

4
checked on Dec 12, 2018

Page view(s)

42
checked on Dec 7, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.