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|Title:||An improved quality control for bisulfite-PCR-based DNA methylation analysis: Cycle threshold value|
|Source:||Ang, P.W., Toh, H.B., Iacopetta, B., Soong, R. (2008-08-01). An improved quality control for bisulfite-PCR-based DNA methylation analysis: Cycle threshold value. Clinical Chemistry and Laboratory Medicine 46 (8) : 1117-1121. ScholarBank@NUS Repository. https://doi.org/10.1515/CCLM.2008.217|
|Abstract:||Background: The diagnostic potential of DNA methylation has generated a need for quality control systems in its analysis. Here, we tested whether cycle threshold (Ct) values from real-time quantitative PCR correlate better with the reliability of bisulfite-PCR methylation analyses than spectrophotometric concentrations. Methods: A total of 18 cell line DNA samples were quantified for β-actin (ACTB) Ct and spectrophotometric values from 1-μL volumes. A further 1 μL was analyzed in quintuplicate for bisulfite DNA ACTB by MethyLight. Correlations between Ct and spectrophotometric values, and MethyLight qualitative (detection in all replicates) and quantitative (Ct value standard deviation|
|Source Title:||Clinical Chemistry and Laboratory Medicine|
|Appears in Collections:||Staff Publications|
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