Please use this identifier to cite or link to this item:
|Title:||Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(e-caprolactone)-tricalcium phosphate-collagen scaffolds||Authors:||Chum, Z.Z.
|Issue Date:||Nov-2009||Citation:||Chum, Z.Z., Woodruff, M.A., Cool, S.M., Hutmacher, D.W. (2009-11). Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(e-caprolactone)-tricalcium phosphate-collagen scaffolds. Acta Biomaterialia 5 (9) : 3305-3315. ScholarBank@NUS Repository. https://doi.org/10.1016/j.actbio.2009.05.015||Abstract:||We evaluate the potential of heparin as a substrate component for the fabrication of bone tissue engineering constructs using poly(e-caprolactone)-tricalcium phosphate-collagen type I (PCL-TCP-Col) three-dimensional (3-D) scaffolds. First we explored the ability of porcine bone marrow precursor cells (MPCs) to differentiate down both the adipogenic and osteogenic pathways within 2-D culture systems, with positive results confirmed by Oil-Red-O and Alizarin Red staining, respectively. Secondly, we examined the influence of heparin on the interaction and behaviour of MPCs when seeded onto PCL-TCP-Col 3-D scaffolds, followed by their induction into the osteogenic lineage. Our 3-D findings suggest that cell metabolism and proliferation increased between days 1 and 14, with deposition of extracellular matrix also observed up to 28 days. However, no noticeable difference could be detected in the extent of osteogenesis for PCL-TCP-Col scaffolds groups with the addition of heparin compared to identical control scaffolds without the addition of heparin. © 2009 Acta Materialia Inc.||Source Title:||Acta Biomaterialia||URI:||http://scholarbank.nus.edu.sg/handle/10635/107927||ISSN:||17427061||DOI:||10.1016/j.actbio.2009.05.015|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 20, 2019
WEB OF SCIENCETM
checked on Jul 10, 2019
checked on Jul 19, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.