Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.actbio.2009.05.015
Title: Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(e-caprolactone)-tricalcium phosphate-collagen scaffolds
Authors: Chum, Z.Z.
Woodruff, M.A.
Cool, S.M. 
Hutmacher, D.W.
Keywords: Cell differentiation
Heparin
PCL-Collagen
Scaffold
Issue Date: Nov-2009
Citation: Chum, Z.Z., Woodruff, M.A., Cool, S.M., Hutmacher, D.W. (2009-11). Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(e-caprolactone)-tricalcium phosphate-collagen scaffolds. Acta Biomaterialia 5 (9) : 3305-3315. ScholarBank@NUS Repository. https://doi.org/10.1016/j.actbio.2009.05.015
Abstract: We evaluate the potential of heparin as a substrate component for the fabrication of bone tissue engineering constructs using poly(e-caprolactone)-tricalcium phosphate-collagen type I (PCL-TCP-Col) three-dimensional (3-D) scaffolds. First we explored the ability of porcine bone marrow precursor cells (MPCs) to differentiate down both the adipogenic and osteogenic pathways within 2-D culture systems, with positive results confirmed by Oil-Red-O and Alizarin Red staining, respectively. Secondly, we examined the influence of heparin on the interaction and behaviour of MPCs when seeded onto PCL-TCP-Col 3-D scaffolds, followed by their induction into the osteogenic lineage. Our 3-D findings suggest that cell metabolism and proliferation increased between days 1 and 14, with deposition of extracellular matrix also observed up to 28 days. However, no noticeable difference could be detected in the extent of osteogenesis for PCL-TCP-Col scaffolds groups with the addition of heparin compared to identical control scaffolds without the addition of heparin. © 2009 Acta Materialia Inc.
Source Title: Acta Biomaterialia
URI: http://scholarbank.nus.edu.sg/handle/10635/107927
ISSN: 17427061
DOI: 10.1016/j.actbio.2009.05.015
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