Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/106930
Title: Ultrastructural in situ hybridization and autoradiographic detection of foreign DNA in zebrafish spermatozoa
Authors: Patil, J.G.
Khoo, H.W. 
Issue Date: 1995
Citation: Patil, J.G.,Khoo, H.W. (1995). Ultrastructural in situ hybridization and autoradiographic detection of foreign DNA in zebrafish spermatozoa. Biochemistry and Molecular Biology International 35 (5) : 965-969. ScholarBank@NUS Repository.
Abstract: Conditions have been established which permit specific hybridization of foreign DNA to complimentary DNA in ultra thin sections of zebrafish sperm prepared for electron microscopy (EM) after fixation in 2% paraformaldehyde, 2.5% glutaraldehyde and embedded in spurs resin. Zebrafish sperm incubated with foreign DNA were subjected to ultrastructural in situ hybridization and autoradiographic detection to determine if the sperm could retain and internalize the foreign DNA sequences. Specific and intense signals were detected from the sperm head indicating that the zebrafish sperm are capable of internalizing foreign DNA.
Source Title: Biochemistry and Molecular Biology International
URI: http://scholarbank.nus.edu.sg/handle/10635/106930
ISSN: 10399712
Appears in Collections:Staff Publications

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