Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/106930
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dc.titleUltrastructural in situ hybridization and autoradiographic detection of foreign DNA in zebrafish spermatozoa
dc.contributor.authorPatil, J.G.
dc.contributor.authorKhoo, H.W.
dc.date.accessioned2014-10-29T07:56:01Z
dc.date.available2014-10-29T07:56:01Z
dc.date.issued1995
dc.identifier.citationPatil, J.G.,Khoo, H.W. (1995). Ultrastructural in situ hybridization and autoradiographic detection of foreign DNA in zebrafish spermatozoa. Biochemistry and Molecular Biology International 35 (5) : 965-969. ScholarBank@NUS Repository.
dc.identifier.issn10399712
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/106930
dc.description.abstractConditions have been established which permit specific hybridization of foreign DNA to complimentary DNA in ultra thin sections of zebrafish sperm prepared for electron microscopy (EM) after fixation in 2% paraformaldehyde, 2.5% glutaraldehyde and embedded in spurs resin. Zebrafish sperm incubated with foreign DNA were subjected to ultrastructural in situ hybridization and autoradiographic detection to determine if the sperm could retain and internalize the foreign DNA sequences. Specific and intense signals were detected from the sperm head indicating that the zebrafish sperm are capable of internalizing foreign DNA.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentZOOLOGY
dc.description.sourcetitleBiochemistry and Molecular Biology International
dc.description.volume35
dc.description.issue5
dc.description.page965-969
dc.description.codenBMBIE
dc.identifier.isiutNOT_IN_WOS
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